CSHL X-Ray Methods in Structural Biology Course

CSHL 结构生物学 X 射线方法课程

• 批准号: 10472057
• 负责人: TERRI I. GRODZICKER
• 金额: $ 1万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10472057
• 关键词: Academia; Area; Automation; Awareness; Biochemistry; Biological; Biological Products; Complement; Complex; Computing Methodologies; Course Content; Cryoelectron Microscopy; Crystallization; Crystallography; Data Collection; Deposition; Development; Discipline; Disease; Disease Progression; Doctor of Medicine; Doctor of Philosophy; Drug Design; Ensure; Faculty; Funding; G-Protein-Coupled Receptors; Generations; Grant; Hand; Harvest; Home; Immersion; Industry; Infrastructure; Inherited; Knowledge; Laboratories; Learning; Lipids; Macromolecular Complexes; Malignant Neoplasms; Maps; Medical Students; Membrane Proteins; Methodology; Methods; Modeling; Modification; Molecular; Molecular Structure; New York; Nobel Prize; Nucleic Acids; Onset of illness; Optics; Participant; Pharmaceutical Preparations; Phase; Postdoctoral Fellow; Procedures; Process; Research; Research Personnel; Resolution; Roentgen Rays; Running; Scientist; Series; Source; Structure; Students; Synchrotrons; Talents; Techniques; Therapeutic antibodies; Time; Training; United States; Universities; Update; Validation; Virus; Work; X ray diffraction analysis; X-Ray Crystallography; base; biological research; computerized data processing; density; design; electron density; experience; experimental study; falls; graduate student; instructor; lecturer; lectures; macromolecule; medical schools; method development; model building; programs; protein structure function; small molecule; structural biology; theories; translational approach; vaccine-induced antibodies; work-study; x-ray free-electron laser

ABSTRACT Cold Spring Harbor Laboratory proposes to continue the course on X-Ray Methods in Structural Biology to be held in the fall of 2017-2021. X-ray methods, spearheaded by X-ray crystallography, yield a wealth of structural information unobtainable through other methods. This intensive 16-day course combines a thorough theoretical approach with practical experience, including extensive laboratory and computational training. Lectures and practical sessions focus on X-ray crystallographic techniques, which remain indispensable in yielding atomic level structural detail of macromolecules, but also extend to X-ray solution scattering (SAXS), and serial crystallography in X-ray free electron lasers (XFEL) and synchrotron sources. The course aspires to provide the opportunity for researchers working in different biological disciplines to become grounded in the techniques and principles of macromolecular crystallography and complementary methods. Learning the theory and practice of the methods at hand, allows participants with a working knowledge of macromolecular structure and function, but who are new to X-ray methods, to harvest the power of recent developments in automation and apply the methods to their current research questions. Topics in X-ray crystallography include basic diffraction theory, crystallization of soluble and also membrane proteins (including lipidic cubic phase techniques), nucleic acids, macromolecular complexes and viruses, crystal cryo-protection and characterization, home, synchrotron, XFEL X-ray sources and optics, X-ray diffraction data collection and data processing, experimental phasing methods including anomalous diffraction, molecular replacement, density modification, electron density map interpretation and model building, structure refinement and validation, as well as coordinate deposition and structure presentation. Topics in X-ray scattering include theory lectures and computational training to derive SAXS invariants and models to understand macromolecular domain organization. Following three decades that have seen two Nobel prize winners graduating from the course, it is time for change: within the duration of the grant, the organization and running of the course will be handed off to a new generation. Two new instructors will participate in the team from the start, and by the end of the grant period a new set of instructors will ensure continuity and renewal. The course will continue to teach participants through extensive hands-on experiments along with lectures and informal discussions on the theory behind the techniques and the latest developments in automating experimental and computational procedures, from world leaders in methods development. Applications are open to a wide range of students including advanced graduate students, medical students, post-doctoral fellows, faculty at universities and medical schools, as well as Ph.D. and M.D. scientists from industry, aiming to allow students to learn techniques that can be immediately incorporated into their own research, also enabling translational approaches including rational drug design.

抽象的 冷泉港实验室提议继续开展结构 X 射线方法课程 生物将于 2017-2021 年秋季举行。 X射线方法，以X射线为先导 晶体学产生了大量通过其他方法无法获得的结构信息。 这个为期 16 天的强化课程将全面的理论方法与实践相结合 经验，包括广泛的实验室和计算培训。讲座及实践 会议重点关注 X 射线晶体学技术，该技术在产量方面仍然不可或缺 大分子的原子级结构细节，还扩展到 X 射线溶液散射 (SAXS) 以及 X 射线自由电子激光器 (XFEL) 和同步加速器中的串行晶体学 来源。该课程致力于为不同领域的研究人员提供机会 生物学科以大分子技术和原理为基础 晶体学和补充方法。学习方法的理论和实践 方便参与者掌握大分子结构和功能的工作知识， 但对于 X 射线方法不熟悉的人，可以收获自动化最新发展的力量 并将这些方法应用于他们当前的研究问题。 X 射线晶体学主题 包括基本衍射理论、可溶性蛋白和膜蛋白的结晶（包括 脂质立方相技术）、核酸、大分子复合物和病毒、晶体 低温保护和表征、家庭、同步加速器、XFEL X 射线源和光学器件、X 射线 衍射数据收集和数据处理，实验定相方法包括 反常衍射、分子置换、密度修正、电子密度图 解释和模型构建、结构细化和验证以及协调 沉积和结构演示。 X 射线散射的主题包括理论讲座和 计算训练以导出 SAXS 不变量和模型以理解大分子 域组织。 三十年来，已有两位诺贝尔奖获得者从该课程毕业， 是时候做出改变了：在资助期限内，课程的组织和运行 将被移交给新一代。两名新教练将加入团队 开始，到资助期结束时，一组新的讲师将确保连续性和 更新。该课程将继续通过广泛的实践实验来教授参与者 以及有关技术背后的理论和最新技术的讲座和非正式讨论 世界领先者在自动化实验和计算程序方面的进展 方法开发。应用程序向广泛的学生开放，包括高级学生 研究生、医学生、博士后、大学和医学界的教师 学校，以及博士学位。和来自工业界的医学博士科学家，旨在让学生学习 可以立即纳入自己的研究中的技术，也使 转化方法，包括合理的药物设计。