PARVOVIRUS INFECTION OF RATS

大鼠细小病毒感染

• 批准号: 2282889
• 负责人: Robert O. Jacoby
• 金额: $ 18.09万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-22 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2282889
• 关键词: Parvoviridae; active immunization; acute disease /disorder; animal age group; antiviral antibody; chronic disease /disorder; communicable disease control; communicable disease transmission; enzyme linked immunosorbent assay; epizootiology; genetic strain; genotype; histochemistry /cytochemistry; immunocytochemistry; in situ hybridization; isolation /quarantine; laboratory rat; latent virus infection; nucleic acid hybridization; organ culture; passive immunization; polymerase chain reaction; serology /serodiagnosis; tissue /cell culture; veterinary medicine; virus antigen; virus diseases; virus infection mechanism; virus replication

Rat virus is a common parvovirus of rats that can cause severe or lethal infection or distort biological responses that require the synthesis of DNA. We have recently shown that inoculation of neonatal or immunodeficient rats leads to persistent infection, whereas inoculation of immunocompetent juvenile rats appears to be shortlived. We also have shown that passively acquired immunity can block persistent-infection. These findings imply that early events determine whether infection is persistent or short-lived and imply that immune,functions may prevent or modulate persistent infection. Aims for continued support stress identification of factors that determine the course and expression of infection and begin to examine strategies for controlling and preventing persistent infection. Experiments will 1) identify early events that influence the course of infection by comparative pathogenesis studies in euthymic and athymic rats, 2) continue characterization of persistent infection using an athymic rat model, 3) determine if infection can be prevented by immunization and 4) determine if host genotype or virus strain influence the expression of infection. These studies will require a variety of techniques including: virus re-isolation and quantification., nucleic acid hybridization in vitro and in situ, serology and immunohistochemistry.

大鼠病毒是大鼠常见的细节病毒，可能导致严重或 致命感染或扭曲需要的生物反应 DNA的合成。 我们最近表明，新生儿或 免疫缺陷大鼠导致持续感染，而接种 免疫能力的少年大鼠似乎很短。 我们也有 表明被动获得的免疫力可以阻止持续感染。 这些发现表明早期事件决定了感染是否是 持续或短暂的，暗示免疫，功能可能预防或 调节持续感染。 旨在持续支持压力的目标 确定决定了决定课程和表达的因素 感染并开始检查控制和预防的策略 持续感染。 实验将1）确定早期事件 通过比较发病机理研究影响感染的过程 安理大鼠和无胸腔大鼠，2）继续表征持久性 使用无胸腺大鼠模型感染，3）确定感染是否可以是 通过免疫预防和4）确定宿主基因型或病毒是 应变影响感染的表达。 这些研究需要 多种技术，包括：病毒再异化和 定量，体外和原位核酸杂交， 血清学和免疫组织化学。

项目成果

Persistent rat parvovirus infection in individually housed rats.

单独饲养的大鼠中持续存在大鼠细小病毒感染。

• DOI: 10.1007/bf01310765
• 发表时间: 1991
• 期刊: Archives of virology
• 影响因子: 2.7
• 作者: Jacoby,RO;Johnson,EA;Paturzo,FX;Gaertner,DJ;Brandsma,JL;Smith,AL
• 通讯作者: Smith,AL

Persistent rat virus infection in juvenile athymic rats and its modulation by immune serum.

幼年无胸腺大鼠的持续性大鼠病毒感染及其免疫血清的调节。

• 发表时间: 1995
• 期刊: Laboratory animal science.
• 作者: Gaertner,DJ;Jacoby,RO;Johnson,EA;Paturzo,FX;Smith,AL
• 通讯作者: Smith,AL

Persistence of rat parvovirus in athymic rats.

大鼠细小病毒在无胸腺大鼠中的持续存在。

• DOI: 10.1007/bf01311362
• 发表时间: 1989
• 期刊: Archives of virology
• 影响因子: 2.7
• 作者: Gaertner,DJ;Jacoby,RO;Smith,AL;Ardito,RB;Paturzo,FX
• 通讯作者: Paturzo,FX

Environmental stability and transmission of rat virus.

环境稳定性和大鼠病毒的传播。

• 发表时间: 1995
• 期刊: Laboratory animal science
• 作者: Yang,FC;Paturzo,FX;Jacoby,RO
• 通讯作者: Jacoby,RO

Efficient induction of persistent and prenatal parvovirus infection in rats.

有效诱导大鼠持续性和产前细小病毒感染。

• DOI: 10.1016/0168-1702(96)01351-2
• 发表时间: 1996
• 期刊: Virus research
• 影响因子: 5
• 作者: Gaertner,DJ;Smith,AL;Jacoby,RO
• 通讯作者: Jacoby,RO