Building Bridges to New Fluorophores

搭建通往新荧光团的桥梁

• 批准号: 10470193
• 负责人: STEPHEN C. MILLER
• 金额: $ 29.77万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-19 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10470193
• 关键词: Acylation; Animals; Area; Binding; Biological; Biology; Blood; Charge; Chemistry; Color; Complex; Development; Dyes; Electrons; Environment; Exhibits; Fluorescein; Fluoresceins; Fluorescence; Fluorescent Dyes; Fluorescent Probes; Goals; Hydroxyl Radical; Light; Liquid substance; Masks; Metals; Modification; Noise; Optics; Oranges; Organelles; Oxides; Peripheral; Phosphines; Phototoxicity; Positioning Attribute; Prize; Property; Proteins; Renaissance; Reporter; Resolution; Rhodamine; Rhodamines; Signal Transduction; Silicon; Site; Sulfonamides; Sulfones; System; Techniques; Tissues; Work; Xanthenes; amino group; analog; attenuation; base; cellular imaging; cyanine; electron donor; fluorophore; in vivo imaging; luciferin; near infrared dye; prevent; redshift; resorufin; scaffold; single molecule; small molecule; virtual

Imaging of cells and tissues using light beyond the visible range is advantageous because there are few endogenous molecules capable of absorbing or emitting at these longer wavelengths. This results in lower phototoxicity and higher signal-to-noise as there is less attenuation of the optical signal and less interference from autofluorescence. Suitable fluorescent dyes in this wavelength region have long been dominated by cyanines, a class of fluorophore that typically has low photostability and large size. The development of smaller and more photostable dyes in this wavelength region is therefore highly desirable. In this proposal, we will explore the scope of electron donors and the incorporation of LUMO-lowering substitutions in different positions and dyes, with the goal of optimized compact near-IR fluorophores, quenchers, and fluorogenic probes for a wide range of applications.

使用超出可见光范围的光对细胞和组织进行成像是有利的，因为 很少有内源性分子能够吸收或发射这些较长的波长。这 由于光信号衰减较小，因此光毒性较低，信噪比较高 自发荧光的干扰较少。该波长范围内合适的荧光染料有 长期以来，花青素占主导地位，花青素是一类通常具有低光稳定性和大荧光团的荧光团。 尺寸。因此，在此波长范围内开发更小、更耐光的染料 非常理想。在本提案中，我们将探讨电子供体的范围和纳入 不同位置和染料的 LUMO 降低取代，目的是优化紧凑型近红外 荧光团、淬灭剂和荧光探针，应用广泛。

项目成果

Silicon functionalization expands the repertoire of Si-rhodamine fluorescent probes.

硅功能化扩展了硅罗丹明荧光探针的种类。

• 发表时间: 2022-05-25
• 影响因子: 8.4
• 作者: Rao, Desaboini Nageswara;Ji, Xincai;Miller, Stephen C
• 通讯作者: Miller, Stephen C