SYNAPTOLOGY OF THE SEPTO-HIPPOCAMPAL COMPLEX

隔海马复合体的联结

• 批准号: 2265790
• 负责人: CSABA LERANTH
• 金额: $ 26.09万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2265790
• 关键词: Cercopithecidae; brain septal area; brain subcortex; calcium binding protein; dentate gyrus; developmental neurobiology; electrical measurement; electron microscopy; electrophysiology; epilepsy; experimental brain lesion; gamma aminobutyrate; glutathione peroxidase; hippocampus; histochemistry /cytochemistry; hypothalamus; innervation; interneurons; laboratory rat; light microscopy; mossy fiber; neural information processing; neuroanatomy; neurochemistry; neuroendocrine system; radiotracer; silver impregnation; substance P; synapses

The overall aim of the proposed studies is to further elucidate the ultrastructural neurochemical anatomy and interconnections of the septo- hippocampal complex. These studies will aid our understanding of mnemonic functions and human diseases, particularly Alzheimer's disease and temporal lobe epilepsy. Additional analyses of the intrinsic and extrinsic connections of the sexually dimorphic and estrogen/androgen sensitive septal complex, particularly with the syndrome and in understanding how the neuroendocrine and cognitive/associational areas of the central nervous system interact. While there are major differences between the human and rodent hippocampal formations, these structures are very similar in the human and monkey brain. Therefore, in addition to rat studies, we propose to continue the analysis of the hippocampal formation of subhuman primates. Two major lines of inquiry are proposed: a) why some hippocampal neurons and areas are more seizure sensitive than others; and b) how the septal complex mediates bidirectional information between the hippocampus and the neuroendocrine hypothalamus. By using interlinked state-of-the-art morphological techniques, including correlated light and electron microscopic double immunostaining methods, antero and retrograde tracer techniques, and Golgi impregnation, as well as by combining electrophysiological and histochemical methods, the following studies are suggested: 1. Further analysis of the synaptology and transmitter content of seizure-sensitive and seizure-resistant neurons in the rat and monkey hippocampal formation. 2. Neurochemistry of interneurons associated with identified mossy cells. 3. Further characterization of extrinsic hippocampal substance P and calretinin systems. 4. Synaptology and neurochemistry of subcortical circuitries controlling septal area neurons and the septo-hippocampal pathway.

拟议研究的总体目的是进一步阐明 超微结构神经化学解剖结构和隔膜的互连 海马复合物。 这些研究将有助于我们对 助记符功能和人类疾病，尤其是阿尔茨海默氏病 和颞叶癫痫。 对内在和的其他分析 性二态和雌激素/雄激素的外在连接 敏感的间隔复合物，尤其是综合征和 了解神经内分泌和认知/关联领域如何 中枢神经系统相互作用。 虽然人与啮齿动物之间存在重大差异 海马形成，这些结构在人类中非常相似 和猴子的大脑。 因此，除了大鼠研究外，我们还建议 继续分析亚人类灵长类动物的海马形成。 提出了两条主要的询问线：a）为什么一些海马神经元 区域比其他地区更敏感。 b）隔隔中的 复杂介导海马和之间的双向信息 神经内分泌下丘脑。 通过使用相关的最新链接 形态学技术，包括相关的光和电子 微观双重免疫染色方法，原始和逆行示踪剂 技术和高尔基浸渍以及通过结合 电生理和组织化学方法，以下研究是 建议： 1。进一步分析突触学和发射器含量的 大鼠和猴子中的癫痫发作敏感和癫痫发作的神经元 海马形成。 2。与已识别的苔藓相关的中间神经元神经化学 细胞。 3。外部海马物质P和 钙网蛋白系统。 4。控制皮层循环的突触学和神经化学 隔膜区域神经元和隔膜海马途径。