GH20-005, Conducting Integrated Infectious Disease and Public Health Research in Peru

GH20-005，在秘鲁进行综合传染病和公共卫生研究

• 批准号: 10462449
• 负责人: Margaret N Kosek
• 金额: $ 24.14万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2025-09-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10462449
• 关键词: GH20; 005; Conducting; Integrated; Infectious

PROJECT SUMMARY: The proposed application aims to overcome diagnostic barriers in the etiologic diagnosis of endemic and epidemic causes of acute febrile illness (AFI) by moving efficient state of the art diagnostics to an area of high endemicity. The long-term goal of this research is to understand the etiology of AFI across diverse contexts in South America and contribute to global strategies that improve etiology-specific disease estimates to inform public health practice and policy. The central hypothesis is that the technology transfer and implementation of improved diagnostics will enhance regional diagnostic capacity, epidemic responsiveness, and translation of research findings to practice maximizing the public health impact of research findings. The rationale for the research is that the documented feasibility of this approach will enable a full and appropriate evaluation for uptake by regional and national stakeholders. The goals of the research will be achieved through the execution of three specific aims: 1) Establish stable year round acute febrile illness surveillance using the TaqMan array card, which simultaneously detects over 30 pathogens of AFI across three epidemiologic contexts in Loreto, Peru; urban, riverine with extensive sylvatic exposure, and agricultural riverine context along the three country border (Peru, Brazil, and Colombia) in order to capture the diversity of pathogens causing AFI in Peru over a four year period; 2) Integrate this diagnostic platform into the regional outbreak response to determine the etiology of epidemics of acute febrile illness and validating improved storage and transport procedures; and 3) Develop and evaluate novel serodiagnostic chip to enable single platform serologic assessment and downstream point of care tests and focused diagnostics for pathogens of high prevalence and importance. The global control of emerging infectious diseases will be improved only as the areas that are not reached and included effectively in surveillance shrink and the proposed project attempts to implement a strategy to make all populations reachable to the improved diagnostics. The complexity of current diagnostic strategies is a critical barrier to the timely regional ascertainment of the etiology of most cases of acute febrile illnesses and this has important regional and global public health implications as undiagnosed AFI allows for the widespread dissemination of emerging diseases as effective disease control measures are based on accurate etiologic assessment. The plan is innovative because of the meaningful integration of highly select local researchers, educators, trainees, and policymakers. The implementation of the research strategy will be significant because it is expected to contribute to the accuracy of etiologic assessment of endemic and epidemic etiologies of AFI in an area of documented diversity of vector-borne and zoonotic agents and serve as a model for enabling local diagnostic and reporting capacity in South America.

项目概要： 所提出的应用旨在克服地方病和流行病病因诊断中的诊断障碍 通过将最先进的高效诊断技术转移到高发地区来解决急性发热性疾病 (AFI) 的流行原因 地方性。这项研究的长期目标是了解不同背景下 AFI 的病因。 南美洲并为改善特定病因疾病估计的全球战略做出贡献，以提供信息 公共卫生实践和政策。中心假设是技术转让和实施 改进的诊断将提高区域诊断能力、流行病应对能力和翻译 研究成果实践最大化研究成果对公共卫生的影响。理由如下： 研究表明，这种方法的可行性记录将有助于对 区域和国家利益攸关方的采纳。研究目标将通过执行来实现 三个具体目标：1) 使用 TaqMan 阵列建立稳定的全年急性发热性疾病监测 卡，可同时检测洛雷托三种流行病学背景下的 30 多种 AFI 病原体， 秘鲁;这三个国家沿线的城市、具有广泛森林暴露的河流以及农业河流环境 边境（秘鲁、巴西和哥伦比亚），以捕获导致秘鲁 AFI 的病原体的多样性 四年期限； 2）将该诊断平台整合到区域疫情应对中，以确定 急性发热性疾病流行病的病因学并验证改进的储存和运输程序；和 3) 开发和评估新型血清诊断芯片，以实现单一平台血清学评估和下游 针对高流行和重要病原体的护理点测试和重点诊断。全局控制 只有未有效覆盖和纳入的地区，新发传染病的发病率才会得到改善 监测范围缩小，拟议项目试图实施一项战略，使所有人口 可以获得改进的诊断。当前诊断策略的复杂性是实现这一目标的关键障碍 及时在区域查明大多数急性发热性疾病病例的病因，这具有重要意义 由于未确诊的 AFI 导致广泛传播，因此对区域和全球公共卫生产生影响 新出现的疾病作为有效的疾病控制措施是基于准确的病因学评估。计划 之所以具有创新性，是因为精心挑选的当地研究人员、教育工作者、实习生和 政策制定者。研究战略的实施将意义重大，因为它预计将有助于 在有记录的地区对 AFI 地方性和流行性病因进行病因学评估的准确性 媒介传播和人畜共患病原体的多样性，并作为实现本地诊断和报告的模型 南美洲的产能。