STRUCTURAL CORRELATES OF CORTICAL INFORMATION PROCESSING

皮质信息处理的结构相关性

• 批准号: 2265055
• 负责人: SHARON L JULIANO
• 金额: $ 15.79万
• 依托单位: HENRY M. JACKSON FDN FOR THE ADV MIL/MED
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2265055
• 关键词: afferent nerve; autoradiography; developmental neurobiology; ferrets; immunocytochemistry; immunofluorescence technique; muscarinic receptor; neocortex; nerve endings; neural plasticity; neuroanatomy; neurogenesis; neuronal guidance; recombinant proteins; somesthetic sensory cortex

The somatosensory cortex is a highly developed region of the brain that demonstrates substantial ability to adapt to environmental changes in both young and adult animals. Although considerable research efforts have identified factors contributing to neuronal plasticity, the precise mechanisms that allow the brain to reorganize have yet to be clearly revealed. It has been known for many years, however, that young brains have a different capacity to adapt after injury or disease than adult brains. The experiments described here will attempt to identify factors that assist in our understanding of why the nervous system possesses a greater reorganizational ability during development than in adulthood. The proposed studies will evaluate the development of ferret somatosensory cortex. The ferret is an excellent animal to use for developmental study since its cerebral cortex is highly immature at birth. First, the normal development of somatosensory cortex will be assessed, followed by evaluating disrupted development produced by lesions to structures identified as being important for normal laminae of the developmental progression. i) The birthdates of neurons destined to reside in different somatosensory cortex and subjacent subplate, as well as their route of migration to the cortex, will be determined using 3H-thymidine injections. The subplate is a region of the developing neocortex that appears consequential in normal, cortical maturation and is identified as a compartment where In ingrowing afferent fiber systems "wait", and possibly interact, before proceeding into the cortex. In addition, the growth of afferent fiber populations into the somatosensory cortex will be studied in the same brains using DiI injections into the cortex, the ventrobasal thalamus, and the basal forebrain. The relationship of these fibers to the maturing cortical and subplate neurons will be analyzed. ii) The distribution of muscarinic receptor subtypes will be studied in the developing somatosensory cortex using antibodies developed against specific recombinant muscarinic receptor proteins. Muscarinic receptors are sensitive to acetylcholine, a substance that modifies neuronal responses and appears to play a significant role in the development of cortical architecture and function. iii) The affect of transecting a peripheral nerve, or a lesion of the subplate, early in life will be assessed for their impact on the organization of cortical layers, the distribution of afferent fibers populations, or the development of muscarinic receptors. I predict that both these lesions will lead to altered cortical function and anatomic organization, and that both systems play an important role in sculpting normal cortical development in the somatosensory system. In addition an understanding of the mechanisms of neural plasticity will be significant in our ability to improve recovery from cerebral vascular accident or trauma to the nervous system.

体感皮质是大脑高度发达的区域 证明了适应环境变化的实质能力 都是年轻动物和成年动物。 虽然大量的研究工作 已经确定了导致神经元可塑性的因素，精确 允许大脑重组的机制尚未清楚 揭示。 然而，已经知道年轻的大脑已有多年了 受伤或疾病后适应能力的能力与成人不同 大脑。 这里描述的实验将尝试识别因素 这有助于我们理解为什么神经系统拥有 发展过程中的重组能力比成年更大。 拟议的研究将评估雪貂的发展 体感皮质。 雪貂是一种出色的动物 发展研究由于其脑皮质高度不成熟 出生。 首先，体感皮质的正常发育将是 评估，然后评估由 对结构的病变被确定为对正常层层很重要的结构 发展进展。 i）神经元的生日 居住在不同的体感皮质和次级子板中 由于它们的迁移途径将使用 3H-胸腺苷注射。 子板是开发的区域 在正常，皮质成熟和 被确定为室内室内的隔间 在进入皮层之前，“等待”，并可能相互作用。在 此外，传入纤维种群的增长到体感中 将使用DII注射到同一大脑中研究皮质 皮质，腹丘脑和基底前脑。 这 这些纤维与成熟的皮质和子板的关系 神经元将进行分析。 ii）毒蕈碱受体的分布 将在开发的体感皮层中研究子类型 针对特定重组毒蕈碱受体开发的抗体 蛋白质。 毒蕈碱受体对乙酰胆碱敏感，A 修饰神经元反应并似乎发挥的物质 在皮质建筑的发展中重要作用 功能。 iii）外周神经或病变的影响 在子板上，将评估生命早期的影响 皮质层的组织，传入纤维的分布 种群或毒蕈碱受体的发展。 我预测 这两个病变都会导致皮质功能和解剖学改变 组织，这两个系统在雕刻中都起着重要作用 体感系统中的正常皮质发育。 另外 了解神经可塑性机制将是显着的 在我们改善脑血管事故中恢复的能力或 神经系统的创伤。