EMBRYONIC AND EARLY POSTNATAL WEAVER MICE

胚胎和产后早期织鼠

• 批准号: 2262005
• 负责人: JAMES H. MILLONIG
• 金额: $ 2.37万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2262005
• 关键词: EMBRYONIC; EARLY; POSTNATAL; WEAVER; MICE

This research proposal combines molecular genetics with neurobiology and cell biology to define the role of the weaver gene in cerebellar development. Mouse microsatellite markers that map close to the gene will be used to identify embryonic weaver homozygotes. This technology will be used to examine the development of the cerebellum in weaver embryos prior to the apparent morphological manifestations of the mutant. In situ and immunocytochemistry analysis will be used to answer these questions. In addition, reaggregate cultures with embryonic weaver granule cells will determine the earliest time in granule cell development that the mutation has an effect. Rescue of these cultures with wild type granule cells will determine the time in cerebellar development that the weaver ligand is present.

该研究提案将分子遗传学与神经生物学和 细胞生物学来定义织布工在小脑中的作用 发展。 映射靠近基因的小鼠微卫星标记 将用于识别胚胎织布纯合子。 这项技术 将用于检查织布工小脑的发展 突变体的明显形态表现之前。 原位和免疫细胞化学分析将用于回答这些 问题。 此外，用胚胎编织重新培养 颗粒细胞将确定颗粒细胞中最早的时间 突变有效的发展。 拯救这些文化 野生型颗粒细胞将决定小脑的时间 织布配体存在的发展。