EMBRYONIC AND EARLY POSTNATAL WEAVER MICE

胚胎和产后早期织鼠

• 批准号: 2262005
• 负责人: JAMES H. MILLONIG
• 金额: $ 2.37万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2262005
• 关键词: EMBRYONIC; EARLY; POSTNATAL; WEAVER; MICE

This research proposal combines molecular genetics with neurobiology and cell biology to define the role of the weaver gene in cerebellar development. Mouse microsatellite markers that map close to the gene will be used to identify embryonic weaver homozygotes. This technology will be used to examine the development of the cerebellum in weaver embryos prior to the apparent morphological manifestations of the mutant. In situ and immunocytochemistry analysis will be used to answer these questions. In addition, reaggregate cultures with embryonic weaver granule cells will determine the earliest time in granule cell development that the mutation has an effect. Rescue of these cultures with wild type granule cells will determine the time in cerebellar development that the weaver ligand is present.

该研究计划将分子遗传学与神经生物学相结合 细胞生物学定义韦弗基因在小脑中的作用 发展。 靠近基因的小鼠微卫星标记 将用于鉴定胚胎织布者纯合子。 这项技术 将用于检查织布者小脑的发育 在突变体出现明显形态学表现之前的胚胎。 原位和免疫细胞化学分析将用于回答这些问题 问题。 此外，用胚胎编织者重新聚集培养物 颗粒细胞将决定颗粒细胞中的最早时间 发展表明突变有影响。 拯救这些文化 野生型颗粒细胞将决定小脑的时间 表明韦弗配体存在。