Integrating Volumetric Light-Field with Computational Fluid Dynamics to Study Myocardial Trabeculation and Function

将体积光场与计算流体动力学相结合来研究心肌小梁和功能

基本信息

批准号：
10458052
负责人：
Tzung K Hsiai
金额：
$ 49.17万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10458052
关键词：
Adult Animal Genetics Animal Model Arrhythmia Biomechanics Budgets Cardiac Cardiac Myocytes Cardiac development Cardiomyopathies Collaborations Congenital Heart Defects Custom Development Diagnosis Diastole Disease ERBB2 gene Event Failure Functional disorder Genetic Genotype Goals Heart Heart failure Hereditary Disease Image Kinetics Knowledge Lasers Left ventricular non-compaction Ligands Light Link Liquid substance Malignant - descriptor Mechanics Mediating Morphogenesis Muscle Cells Mutation Myocardial Myocardial Contraction Myocardium Myopathy Non-compaction cardiomyopathy Optics Oxygen Pathway interactions Patients Perfusion Persons Pharmacology Phenotype Prevalence Resolution Signal Transduction Structure System Testing Time United States Ventricular Ventricular Dysfunction Ventricular Function Ventricular Remodeling Zebrafish automated segmentation deep learning erbB-2 Receptor gene interaction hemodynamics high risk in silico indexing insight interdisciplinary approach learning strategy mechanotransduction multidisciplinary notch protein preservation receptor shear stress skeletal vector

项目摘要

ABSTRACT Integrating Volumetric Light-Field with Computational Fluid Dynamics to Study Myocardial Trabeculation and Function Non-compaction cardiomyopathy (NCC) is a disease of endomyocardial trabeculation or known as spongy myocardium. NCC carries a high risk of malignant arrhythmias, thromboembolic events, and ventricular dysfunction in association with congenital heart defects or skeletal myopathy. Studies have linked left ventricular non-compaction with autosomal dominant inherited disorders, and mutations in Notch pathways are implicated in defective trabeculation and ventricular NCC. Biomechanical force is intimately connected with mechanotransduction and cardiac morphogenesis. During development, the myocardium differentiates into an outer compact zone and an inner trabeculated zone. Notch receptor- ligand interaction induces EphrinB2-Nrg-ErbB2 signaling to initiate trabecular formation. Our in silico analysis (Alison Marsden, Stanford) revealed elevated oscillatory shear index (OSI) in trabecular ridges, leading to increased viscous dissipation, which was associated with changes in ventricular contractile function and remodeling. However, uncoupling myocardial contraction from intracardiac flow dynamics to elucidate Notch-mediated trabecular organization and subsequent associated changes in local hemodynamics remains an unmet biomechanical challenge. In this context, we hypothesize that hemodynamic shear and myocardial contractile forces coordinate trabecular organization needed to preserve the ventricular structure and contractile efficiency. In combination of laser light-sheet and light- field for super resolution and volumetric imaging, we simultaneously captured myocardial contraction and intracardiac flow dynamics. In collaboration with Stanford Cardiac Mechanics, we integrated fluid structure interaction (FSI) with super resolution imaging to demonstrate 4-D endocardial shear stress in the trabecular ridges and grooves as possible developmental modulator. To test our hypothesis, we propose three specific aims. In Aim 1, we will demonstrate that intracardiac shear stress activates endocardial Delta-Notch signaling to promote trabecular ridge formation. In Aim 2, we will demonstrate that ventricular contraction activates myocardial Jagged-Notch signaling to organize trabecular groove formation. In Aim 3, we will demonstrate that the combination of trabecular ridge and groove formation leads to optimal local hemodynamics and ventricular energetics. The integration of advanced imaging, fluid structure interaction, and zebrafish genetics is uniquely suitable to unveil trabecular organization in relation to kinetic energy dissipation. Our multi-disciplinary approach provides new biomechanical insights into non-compaction cardiomyopathy with pathophysiological significance to ventricular remodeling and function.

抽象的将体积光场与计算流体动力学相结合来研究心肌小梁形成和功能非致密化心肌病 (NCC) 是一种心内膜心肌小梁疾病，或称为海绵状心肌。 NCC 具有发生恶性心律失常、血栓栓塞事件和与先天性心脏缺陷或骨骼肌病相关的心室功能障碍。研究有左心室致密化不全与常染色体显性遗传性疾病以及基因突变有关 Notch 通路与小梁缺陷和心室 NCC 相关。生物力学力是与机械转导和心脏形态发生密切相关。在开发过程中，心肌分化为外部致密区和内部小梁区。 Notch受体- 配体相互作用诱导 EphrinB2-Nrg-ErbB2 信号传导启动小梁形成。我们的计算机分析（Alison Marsden，斯坦福大学）显示小梁中的振荡剪切指数 (OSI) 升高脊，导致粘性耗散增加，这与心室的变化有关收缩功能和重塑。然而，将心肌收缩与心内血流脱钩动力学来阐明Notch介导的小梁组织和随后的相关变化局部血流动力学仍然是一个未解决的生物力学挑战。在这种背景下，我们假设血流动力学剪切力和心肌收缩力协调所需的小梁组织保持心室结构和收缩效率。结合激光光片和光在超分辨率和体积成像领域，我们同时捕获了心肌收缩和心内血流动力学。与斯坦福心脏力学合作，我们集成了流体结构相互作用 (FSI) 与超分辨率成像可展示 4-D 心内膜剪切应力小梁脊和凹槽作为可能的发育调节器。为了检验我们的假设，我们提出三个具体目标。在目标 1 中，我们将证明心内剪切应力激活心内膜 Delta-Notch 信号传导促进小梁嵴形成。在目标 2 中，我们将证明心室收缩激活心肌锯齿状切迹信号以组织小梁沟的形成。在目标 3 中，我们将证明小梁脊的组合凹槽的形成导致最佳的局部血流动力学和心室能量学。这先进成像、流体结构相互作用和斑马鱼遗传学的整合非常适合揭示与动能耗散相关的小梁组织。我们的多学科方法为非致密化心肌病的病理生理学提供了新的生物力学见解对心室重构和功能具有重要意义。