DNA FINGERPRINTING OF TUBERCULOSIS

结核病的 DNA 指纹图谱

• 批准号: 2057197
• 负责人: GARY L TEMPLETON
• 金额: $ 7.83万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2057197
• 关键词: Mycobacterium tuberculosis; genetic strain; human tissue; microorganism classification; respiratory disorder epidemiology; restriction fragment length polymorphism; tuberculosis

The basic research in this proposal will be conducted in the Mycobacteriology Lab at the Little Rock, VA Hospital, where DNA fingerprinting of M. tuberculosis was developed. In the proposed study, all of the Mycobacterium tuberculosis isolated from persons in Arkansas over a period of three years will be analyzed by a recently developed DNA fingerprinting method. Clinical and demographic data will be collected for each patient by the Tuberculosis Control Division of the Arkansas State Department of Health. The specific aims of the research proposal are as follows: 1. Determine the frequency if identical DNA fingerprint patterns among strains of M. tuberculosis when there is no known epidemiologic link. 2. Determine the percentage of tuberculosis in the general population that is due to reactivated infection. 3. Determine the extent of primary tuberculosis in residents and employees of prisons, nursing homes, hospitals and shelters for the homeless. 4. Determine the extent to which nursing homes, prisons, hospitals and homeless shelters are a source of M. tuberculosis infection of people that live and work outside these institutions. 5. Determine whether certain DNA fingerprint patterns are associated with specific clinical presentation of tuberculosis. 6. Develop a more refined M. tuberculosis fingerprinting technique using segments of DNA which are frequently found flanking the insertion sequence IS6110.

该提案的基础研究将在 弗吉尼亚州小石城的分枝杆菌实验室，DNA 开发了结核分枝杆菌的指纹。 在拟议的研究中 从阿肯色州的人分离出的所有结核分枝杆菌 在三年的时间内将通过最近开发的DNA分析 指纹方法。 将收集临床和人口统计数据 对于每个患者，阿肯色州的结核病控制部门 州卫生部。 研究建议的具体目的 如下： 1。确定频率是否相同的DNA指纹图案 当没有已知的流行病学联系时，结核分枝杆菌的菌株。 2。确定一般人群中结核病的百分比 这是由于感染重新激活的。 3。确定居民的原发性结核病的程度 监狱，疗养院，医院和庇护所的员工 无家可归。 4。确定疗养院，监狱，医院和 无家可归的庇护所是结核分枝杆菌感染的来源 在这些机构之外生活和工作。 5。确定某些DNA指纹模式是否与 结核病的特定临床表现。 6。使用更精致的结核分枝杆菌指纹技术使用 经常发现插入侧面的DNA段 序列IS6110。