MODULAR ULTRAHIGH THROUGHPUT DNA SEQUENCING

模块化超高通量 DNA 测序

• 批准号: 2209184
• 负责人: Gregory T. Went
• 金额: $ 33.49万
• 依托单位: CURAGEN CORPORATION
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-25 至 1997-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2209184
• 关键词: DNA; argon; biomedical automation; biomedical equipment development; charge coupled device camera; computer assisted sequence analysis; computer system design /evaluation; fluorescence spectrometry; gel electrophoresis; lasers; nucleic acid sequence; personal computers; polymerase chain reaction

Improving the speed and efficiency of DNA sequencing is essential to the success of the Human Genome Project and to the success of many biotechnology companies. Limitations due to the speed and cost of DNA sequencing are inhibiting new ways of integrating molecular biology and biomedical research to reduce health care costs. Our goal is dramatically to increase the speed and efficiency of Sanger sequencing primarily through adoption of microfabrication techniques. We will create a working prototype microfabricated separation module, load interfacing protocol, and software which can be integrated with our existing modular optical platform. This will allow analysis of 96 samples within four hours: from genomic or other unpurified DNA sources to proofread sequence data. CuraGen has expertise in the physical, computational and biological sciences, and collaborates with the National Nanofabrication Facility and medical community. This combines with the coexistence of instrument/software development and application-driven sequencing demand to put us in a unique position to conceive and carry out this effort. Phase III commercial potential accrues not only from the current market for DNA sequencing capability, buy also from entirely new pharmaceutical, diagnostic and clinical markets which a reliable, high-throughput low-cost device would enable. PROPOSED COMMERCIAL APPLICATION: The development of a modular DNA sequencing machine with ultrahigh throughput would make possible simultaneous reductions in reagent, personnel and instrument costs. Such a development would have enormous commercial potential in its own right. This value will be greatly outweighed by the impact on the health care industry of the data generated by use in medical research. The modular nature of the instrument proposed here will add value to the existing instrument base.

提高DNA测序的速度和效率对于 人类基因组项目的成功和许多人的成功 生物技术公司。 由于DNA的速度和成本而导致的限制 测序抑制了整合分子生物学和 生物医学研究以降低医疗保健成本。 我们的目标是 急剧提高Sanger测序的速度和效率 主要是通过采用微加工技术。 我们将 创建一个工作原型的微型分离模块，负载 接口协议和可以与我们的软件集成 现有的模块化光学平台。 这将允许分析96 样品在四个小时内：来自基因组或其他未纯的DNA来源 校对序列数据。 Curagen在物理上有专业知识 计算和生物科学，并与国家合作 纳米制造设施和医疗界。 这与 仪器/软件开发和应用程序驱动的共存 测序要求我们处于构想和携带的独特位置 做出这项努力。 第三阶段的商业潜力不仅从 当前的DNA测序能力市场，也完全从 新的药物，诊断和临床市场，这是一个可靠的， 高通量低成本设备将启用。 拟议的商业应用：模块化DNA的发展 用超高吞吐量的测序机将成为可能 同时减少试剂，人员和仪器成本。 这样的 发展本身将具有巨大的商业潜力。 对医疗保健的影响将大大超过这种价值 在医学研究中使用的数据行业。 模块化 这里提出的仪器的性质将为现有的价值增加价值 仪器基础。