Mouse Models of Fibroproliferative Lung Disease

纤维增生性肺病小鼠模型

基本信息

批准号：
10450039
负责人：
Dianhua Jiang
金额：
$ 42.5万
依托单位：
CEDARS-SINAI MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-08-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10450039
关键词：
Adhesions Allografting Animal Experimentation Animal Model Animals Apoptosis Biological Biological Assay Bleomycin Bronchoalveolar Lavage Fluid CD44 gene Cell Line Cells Chronic Collagen Collection Computers Data Databases Deposition Development Disease model Doctor of Philosophy Ensure Epithelial Epithelial Cells Equipment Fibroblasts Fibrosis Functional disorder Funding Gene Deletion Goals Grant HAS2 gene Harvest Histologic Histology Human Hyaluronan Immune Immune response In Vitro Inflammation Injections Injury Knowledge Lung Lung Transplantation Lung diseases Measurement Medical center Medicine Mesenchymal Messenger RNA Methods Modeling Molecular Mouse Strains Mus Organoids Outcome Pathogenesis Patients Performance Phenotype Play Population Analysis Primary Cell Cultures Procedures Process Productivity Proteins Protocols documentation Publications Pulmonary Fibrosis Pulmonary Inflammation Research Research Personnel Resources Role SCID Mice Sampling Specimen Stains Standardization Structure of parenchyma of lung Study models Tamoxifen Techniques Time Tissue Banks Tissue Harvesting Tissues Training Work airway remodeling cost epithelial stem cell experience experimental study in vivo interstitial lung allograft lung injury macrophage man matrigel migration mouse model novel professor programs success

项目摘要

ABSTRACT The purpose of Core B - Mouse Models of Fibroproliferative Lung Disease and Sample Core – is to provide a resource for the Program investigators to conduct studies in both in vivo studies with mouse models and in vitro primary cell culture models. The Animal Core will be led by Dianhua Jiang, M.D, Ph.D., Professor of Medicine at Cedars-Sinai Medical Center, who is currently a PI of the Animal Core for the PPG program in the past years and has successfully supported all the projects. During the last funding period, the animal core has successfully supported the three projects and achieved the proposed goals, and generated multiple mouse strains required by all different projects and performed mouse modeling studies of lung fibrosis, resulting in several high impact publications and forming the basis for renewal of this Program grant. The responsibility of the Core includes providing training, standard operating procedures and facilitating for mouse models of interstitial fibrosis and CLAD and developing a core unit to support these projects. Core B will unify the three Projects by standardizing the aspects of animal experimentation and promote interactions among those involved in the Projects. Core B will conduct treatments required to induce fibroproliferative lung disease in mice and be responsible for outcome measurement. Fibroproliferative lung disease models will include intratracheal bleomycin, mouse model of CLAD, and human lung fibroblasts from IPF patients to immune compromised mice (SCID mice). Core B will isolate, fix and process mouse lung tissues and will perform standard histology staining for lung inflammation and fibrosis measurement. Additionally, the Core will serve to standardize histological scoring by implementing computer-automated assessment of lung hyaluronan, collagen, airway remodeling, epithelial cell apoptosis and inflammation. Core B will isolate, maintain and provide program investigators with primary cell lines including lung fibroblasts, macrophages and epithelial cells from mouse models. This facility serves to standardize culture methods across the three projects. The Core will also generate and maintain a mouse strain and results database, organize a tissue repository and histopathologic analysis. Centralizing the animal work in the Animal Core will reduce the cost of producing high-quality data and will facilitate interactions among the projects. Therefore, the Animal Core will play an important role in unifying the three Projects by standardizing all aspects of animal experimentation and promote interactions among investigators involved in the Projects to ensure the successful completion of studies proposed in this Program Project.

抽象的核心 B - 纤维增生性肺病小鼠模型和样本核心 - 的目的是提供为该计划研究人员提供小鼠模型体内研究和体内研究的资源体外原代细胞培养模型将由 Dianhua Jiang 博士、教授领导。 Cedars-Sinai 医疗中心的医学博士，目前是该地区 PPG 项目动物核心的 PI 在过去的几年里，动物核心已经成功支持了所有项目。成功支持三个项目并实现拟定目标，并产生多只小鼠所有不同项目所需的菌株并进行了肺纤维化的小鼠模型研究，结果几份高影响力的出版物，并构成续订该计划拨款的基础。核心包括提供培训、标准操作程序和促进小鼠模型的建立间质纤维化和 CLAD 并开发一个核心单元来支持这些项目将统一这三个项目。通过标准化动物实验方面并促进这些方面之间的互动的项目参与该项目的 Core B 将进行诱导纤维增生性肺病所需的治疗。小鼠并负责结果测量，其中包括纤维增殖性肺病模型。气管内博莱霉素、CLAD小鼠模型以及IPF患者的人肺成纤维细胞进行免疫受损小鼠（SCID 小鼠）将分离、修复和处理小鼠肺组织并执行操作。此外，Core 将用于肺部炎症和纤维化测量的标准组织学染色。通过对肺透明质酸进行计算机自动评估来标准化组织学评分， Core B 将隔离、维持和炎症。为项目研究人员提供原代细胞系，包括肺成纤维细胞、巨噬细胞和上皮细胞该设施用于标准化三个项目的培养方法。 Core 还将生成并维护小鼠品系和结果数据库，组织组织存储库并将动物工作集中在动物核心中将降低生产成本。高质量的数据，并将促进项目之间的互动，因此，动物核心将发挥作用。通过标准化动物实验和实验的各个方面，在统一这三个项目方面发挥着重要作用促进参与项目的研究人员之间的互动，以确保项目的成功完成本计划项目中提出的研究。