CSFS--BIOLOGICAL EFFECTS ON CELLS OF NEURAL CREST ORIGIN

CSFS--对神经嵴起源细胞的生物学作用

• 批准号: 2272254
• 负责人: GAYLE COCITA BALDWIN
• 金额: $ 9.56万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-04-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2272254
• 关键词: SCID mouse; biopsy; cell growth regulation; colony stimulating factor; cytokine receptors; developmental neurobiology; embryogenesis; growth factor receptors; human subject; in situ hybridization; interleukin 1; melanoma; metastasis; myelination; neoplastic cell; neural crest; neuroblastoma; oligodendroglia; polymerase chain reaction; protein structure function; receptor binding; receptor expression; stainings; tissue /cell culture; tumor necrosis factor alpha

DESCRIPTION: (Adapted from applicant's abstract) Hematopoietic growth factors (colony-stimulating factors or CSFs) are known to stimulate the proliferation and maturation of blood cells and their precursors. While the clinical applications of CSFs in stimulating proliferation and maturation of blood cells are well described, its role in effecting non-hematopoietic tumor cell growth remains poorly understood. Additionally, CSFs may have functional effects on the development of non-hematopoietic tissues, particularly those of neural cell origin. The goal of this application is to define and characterize the regulatory effects of two specific human CSFs on non- hematopoietic cells (i.e., granulocyte-macrophage colony-stimulating factor [GM-CSF] and granulocyte colony-stimulating factor [G-CSF]). These studies will determine GM-CSF and G-CSF responsiveness and receptor expression on primary human melanoma and neuroblastomas in both in vitro clonogenic assays systems, as well as in vivo transplantation studies using human tumor cells in severe combined immune deficiency (SCID) mice. The second goal of this proposal is to characterize a functional role of GM-CSF, G-CSF, and their respective receptors in the development of the central nervous system and in neurological disorders.

描述：（改编自申请人的摘要）造血生长 已知因子（集落刺激因子或 CSF）可刺激 血细胞及其前体的增殖和成熟。 而CSF在刺激增殖方面的临床应用 血细胞的成熟和成熟已得到很好的描述，其在 对非造血肿瘤细胞生长的影响仍然很差 明白了。 此外，CSF 可能对 非造血组织的发育，特别是 神经细胞起源。 该应用程序的目标是定义和 描述两种特定人类 CSF 对非 造血细胞（即粒细胞-巨噬细胞集落刺激 因子[GM-CSF]和粒细胞集落刺激因子[G-CSF]）。 这些研究将确定 GM-CSF 和 G-CSF 的反应性以及 原发性人黑色素瘤和神经母细胞瘤中受体的表达 体外克隆形成分析系统以及体内 使用人类肿瘤细胞进行严重联合移植研究 免疫缺陷（SCID）小鼠。 该提案的第二个目标是 描述 GM-CSF、G-CSF 及其各自的功能作用 中枢神经系统发育中的各个受体 以及神经系统疾病。