CSFS--BIOLOGICAL EFFECTS ON CELLS OF NEURAL CREST ORIGIN

CSFS--对神经嵴起源细胞的生物学作用

• 批准号: 2272254
• 负责人: GAYLE COCITA BALDWIN
• 金额: $ 9.56万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-04-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2272254
• 关键词: SCID mouse; biopsy; cell growth regulation; colony stimulating factor; cytokine receptors; developmental neurobiology; embryogenesis; growth factor receptors; human subject; in situ hybridization; interleukin 1; melanoma; metastasis; myelination; neoplastic cell; neural crest; neuroblastoma; oligodendroglia; polymerase chain reaction; protein structure function; receptor binding; receptor expression; stainings; tissue /cell culture; tumor necrosis factor alpha

DESCRIPTION: (Adapted from applicant's abstract) Hematopoietic growth factors (colony-stimulating factors or CSFs) are known to stimulate the proliferation and maturation of blood cells and their precursors. While the clinical applications of CSFs in stimulating proliferation and maturation of blood cells are well described, its role in effecting non-hematopoietic tumor cell growth remains poorly understood. Additionally, CSFs may have functional effects on the development of non-hematopoietic tissues, particularly those of neural cell origin. The goal of this application is to define and characterize the regulatory effects of two specific human CSFs on non- hematopoietic cells (i.e., granulocyte-macrophage colony-stimulating factor [GM-CSF] and granulocyte colony-stimulating factor [G-CSF]). These studies will determine GM-CSF and G-CSF responsiveness and receptor expression on primary human melanoma and neuroblastomas in both in vitro clonogenic assays systems, as well as in vivo transplantation studies using human tumor cells in severe combined immune deficiency (SCID) mice. The second goal of this proposal is to characterize a functional role of GM-CSF, G-CSF, and their respective receptors in the development of the central nervous system and in neurological disorders.

描述：（改编自申请人的摘要）造血生长 已知因素（刺激因子或CSF）刺激 血细胞及其前体的增殖和成熟。 而CSF在刺激增殖中的临床应用 充分描述了血细胞的成熟，其在 影响非脊髓性肿瘤细胞的生长仍然很差 理解。 此外，CSF可能对 开发非造成杂草组织，尤其是 神经细胞起源。 此应用的目的是定义和 表征了两个特定人类企业对非 - 的调节作用 造血细胞（即粒细胞 - 巨噬细胞刺激刺激 因子[GM-CSF]和粒细胞群刺激因子[G-CSF]）。 这些研究将确定GM-CSF和G-CSF的响应能力以及 原发性人黑色素瘤和神经母细胞瘤的受体表达 两者都在体外克隆化测定系统以及体内 使用人类肿瘤细胞在严重的合并中使用人类肿瘤细胞的移植研究 免疫缺陷（SCID）小鼠。 该提议的第二个目标是 表征GM-CSF，G-CSF及其功能的作用 中枢神经系统发展中的各自受体 和神经系统疾病。