Study of Interleukin 33 as a new immunotherapy of lung cancer

白细胞介素33作为肺癌新型免疫疗法的研究

基本信息

批准号：
10442707
负责人：
Song Li
金额：
$ 8.14万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10442707
关键词：
Affect Bioinformatics CD4 Positive T Lymphocytes CD8-Positive T-Lymphocytes CD8B1 gene CTLA4 gene Cancer Patient Cell Nucleus Cells Combination Drug Therapy Data Development Down-Regulation Epithelial Epithelial Cells Family Flow Cytometry Genes Genetic Transcription Histologic Human Immune Immune Tolerance Immune response Immunologic Surveillance Immunotherapy Infection Inflammation Interleukin-1 Interleukins Link Lung Lung Adenocarcinoma Lung Neoplasms Malignant - descriptor Malignant Neoplasms Malignant neoplasm of lung Mediating Microscopy Modality Modeling Molecular Monoclonal Antibodies Mus Mutate Mutation Paclitaxel Plasmids Regulatory T-Lymphocyte Resistance Role Signal Transduction Signaling Molecule Site Solid Neoplasm TP53 gene Testing Th1 Cells The Cancer Genome Atlas Therapeutic Therapeutic Effect Tissues Transgenic Mice Translations Treatment Efficacy Tumor Escape Tumor Immunity Tumor Suppression Tumor Suppressor Genes Tumor Tissue Work anti-CTLA4 anti-PD-1 anti-tumor immune response base cancer immunotherapy cancer survival cancer therapy cell mediated immune response chemotherapy clinically relevant cytokine cytotoxic gain of function gene delivery system gene therapy genetic approach high dimensionality immune checkpoint blockade immunogenic improved loss of function lung cancer cell lung tumorigenesis member mouse model neoplasm immunotherapy neoplastic cell novel overexpression programmed cell death protein 1 resistance mechanism response systemic toxicity tumor tumor growth tumor microenvironment tumor progression tumorigenesis

项目摘要

The immune suppressive tumor microenvironment (TME) has limited the response rate of Immune checkpoint blockade (ICB) therapy. Lack of immune stimulatory “danger” signal molecules in TME can be a major factor contributing to resistance to tumor immunotherapy. Therefore, reprogramming the TME by over-expression of alarmin represents a novel and promising tumor immunotherapy. We have found that IL-33, which is a “danger” signal, is expressed in normal lung epithelial cells but drastically down-regulated in human malignant lung cancer cells. Our bioinformatics analysis of the TCGA data has suggested that loss of tumor protein p53 (TP53) is responsible for IL-33 down-regulation in epithelial cells during tumorigenesis of multiple human cancers. In addition, IL-33 expression was reduced in p53 deficient compared to p53 WT murine lung tumor cells. These data suggest that down-regulation of IL-33 is a major mechanism of tumor immune evasion. Using mouse tumor models, we demonstrated that IL-33 was highly expressed in immunogenic murine tumor cells and its level could be further increased during ICB tumor therapy. Importantly, we showed that the ST2/IL-33R signaling was required for therapeutic effect of ICB therapy. Furthermore, we showed that tumor tissue expression of IL-33 synergized with both chemotherapy and ICB in inhibiting lung tumor growth. And the increased therapeutic efficacy was associated with increases in function and number of CD103+CD8+ T cells as well as decreases in Treg. We hypothesize that a novel immunotherapy based on reprogramming TME by “alarmin” IL-33 synergizes with chemotherapy and ICB immunotherapy through promoting tumoral CD103+CD8+ T cells. SA1. Test the hypothesis that loss of the tumor suppressor gene p53 causes the down- regulation of epithelial IL-33 expression, leading to reduced tumor immune surveillance of lung tumor and accelerated lung tumor progression. SA2. We will focus on development of an improved therapy that is based on codelivery of IL-33 expressing plasmid and paclitaxel (PTX), alone or in combination with either PD1 mAbs or CTLA-4 mAbs-based ICB.

免疫抑制性肿瘤微环境（TME）限制了免疫检查点的反应率 TME 中缺乏免疫刺激“危险”信号分子可能是一个主要因素。因此，通过过表达 TME 来重新编程。 Alarmin 代表了一种新颖且有前途的肿瘤免疫疗法。 “危险”信号在正常肺上皮细胞中表达，但在人类恶性肿瘤中显着下调我们对 TCGA 数据的生物信息学分析表明肿瘤蛋白 p53 丢失。 (TP53) 负责多种人类肿瘤发生过程中上皮细胞中 IL-33 的下调此外，与 p53 WT 小鼠肺肿瘤相比，p53 缺陷的 IL-33 表达降低。这些数据表明 IL-33 的下调是肿瘤免疫逃避的主要机制。小鼠肿瘤模型中，我们证明 IL-33 在免疫原性小鼠肿瘤细胞中高表达重要的是，我们发现 ST2/IL-33R 在 ICB 肿瘤治疗期间可能会进一步增加。 ICB 疗法的治疗效果需要信号传导。此外，我们还发现肿瘤组织。 IL-33 的表达与化疗和 ICB 协同抑制肺肿瘤生长。治疗效果的提高与 CD103+CD8+ T 细胞功能和数量的增加相关以及 Treg 的减少，我们勇敢地提出了一种基于 TME 重编程的新型免疫疗法。 “警报素”IL-33 通过促进肿瘤细胞与化疗和 ICB 免疫治疗产生协同作用 CD103+CD8+ T 细胞 SA1 的缺失导致肿瘤抑制基因 p53 的下降。调节上皮 IL-33 表达，导致肺肿瘤的肿瘤免疫监视减少，我们将重点开发基于 SA2 的改进疗法。单独或与 PD1 单克隆抗体联合递送 IL-33 表达质粒和紫杉醇 (PTX) 或基于 CTLA-4 mAb 的 ICB。