Tick Resources Core

勾选资源核心

基本信息

批准号：
10440406
负责人：
Ulrike Gertrud Munderloh
金额：
$ 19.25万
依托单位：
UNIV OF MARYLAND, COLLEGE PARK
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-13 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10440406
关键词：
Adult American Type Culture Collection Anaplasma phagocytophilum Animal Model Arthropods Basic Science Biochemical Biological Assay Black-legged Tick Blood Borrelia burgdorferi Cell Culture Techniques Cell Line Cells Clustered Regularly Interspaced Short Palindromic Repeats Collaborations Collection Communities Complement Contracts Deposition Development Entomology Exposure to Genes Genetic Genome Goals Immune Immune response Immune signaling Immune system Immunity Immunologics Immunology In Vitro Infection Investigation Knock-out Knowledge Laboratories Mass Spectrum Analysis Membrane Microbiology Minnesota Modification Molecular Analysis Mutagenesis National Institute of Allergy and Infectious Disease Nymph Oral Pathogenesis Pathway interactions Peptides Plasmids Proteomics RNA Interference RNA delivery Reagent Reporter Research Research Personnel Research Project Grants Research Support Resources Rickettsia Source System Techniques Testing Ticks Training Transcript Translational Research Universities Work base biodefense cost differential expression efficacy testing functional genomics gene product genome editing germ free condition immune function in vivo insight member microbial microbiota microorganism interaction new technology novel nuclease overexpression pathogen professor repository response symbiont tool transcriptomics vector tick web site

项目摘要

TICK RESOURCES CORE Abstract: The overarching goal of the Tick Resources Core within this P01 application is to provide support to all three research projects and share reagents, materials and new technologies with the scientific community. Requests from the community will be handled through the Biodefense and Emerging Infections Research Resources Repository (BEI) website (www.beiresources.org). BEI was established by the National Institute of Allergy and Infectious Diseases and has been managed under contract by the American Type Culture Collection for fifteen years. Dr. Ulrike Munderloh, a Professor at the University of Minnesota, will be the director of this technical core and is ideally suited to manage the facility. First, she has been a pioneer in the field of tick-pathogen interactions by developing and refining techniques for mutagenesis, plasmid-based complementation, and functional genomics for rickettsial agents in addition to inventing a membrane feeder system for ticks. Second, her laboratory has contributed to 22 out of 30 Rickettsiales species for sequencing in the “Rickettsiales Genomes” project, and supplied 10 of the 14 isolates deposited in BEI. Third, Dr. Munderloh has an outstanding record of distributing tick cell lines and microbial isolates to the scientific community. Aim#1 of the Tick Resources Core will provide currently available tick-vector-based tools to support the research for all three research projects within this P01 application. Aim#2 of the Tick Resources Core will develop novel cell lines suitable for analyses of immune pathways that are activated or repressed in response to pathogens, symbionts and selected microbiota. This will be achieved through RNA interference, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), reporter tags and overexpression assays. Model organisms have a myriad of tools that facilitate the study of pathogen-arthropod interactions. They also provide critical insights to the field of entomology, microbiology and immunology. However, we present strong evidence in this P01 application that Ixodes scapularis ticks seem to be built on entirely different principles that govern microbial pathogenesis and immunity. Therefore, a strong Tick Resources Core is of utmost importance to support all three Projects within this P01 application. By providing existing I. scapularis-based tools and developing novel technologies to investigators, the Tick Resources Core will contribute to uncover novel paradigms in I. scapularis-microbial interactions. This scientific knowledge has potential implications for basic and translational science.

Tick 资源核心摘要：此 P01 应用程序中 Tick 资源核心的总体目标是为所有三个研究项目并与科学界分享试剂、材料和新技术。社区的请求将通过生物防御和新发感染研究来处理资源库（BEI）网站（www.beiresources.org）由国家研究所建立。过敏和传染病，并由美国典型文化公司签约管理明尼苏达大学教授 Ulrike Munderloh 博士将担任该藏品的馆长。首先，她是该技术核心领域的先驱，非常适合管理该设施。通过开发和完善基于质粒的诱变技术来实现蜱与病原体的相互作用除了发明膜饲养器之外，还研究了立克次体病原体的互补和功能基因组学其次，她的实验室对 30 种立克次氏体中的 22 种进行了测序。 “立克次体基因组”项目，并提供了 BEI 保藏的 14 个分离株中的 10 个。第三，Munderloh 博士。在向科学界分发蜱细胞系和微生物分离株方面拥有出色的记录 Aim#1。蜱资源核心将提供当前可用的基于蜱向量的工具来支持以下研究： Tick 资源核心的 P01 应用程序中的所有三个研究项目都将开发新型细胞。适合分析响应病原体而激活或抑制的免疫途径的细胞系，这将通过 RNA 干扰、定期聚类来实现。间隔短回文重复序列 (CRISPR/Cas9)、报告标签和过表达分析。生物体有无数的工具可以促进病原体与节肢动物相互作用的研究。然而，我们提供了强有力的证据。在这个 P01 应用中，肩胛硬蜱似乎是建立在完全不同的控制原理之上的因此，强大的蜱资源核心对于微生物发病机制和免疫至关重要。通过提供现有的基于肩胛骨的工具和支持此 P01 应用程序中的所有三个项目。 Tick 资源核心为研究人员开发新技术，将有助于发现新颖的技术肩胛肌-微生物相互作用的范例这种科学知识对基础知识具有潜在的影响。和转化科学。