DEVELOPMENT OF BIOLOGICALLY INTEGRATABLE TITANIUM IMPLANTS

生物可集成钛植入物的开发

• 批准号: 3732418
• 负责人: KIM R MELTON
• 金额: --
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3732418
• 关键词: autoradiography; biomaterial compatibility; biomaterial development /preparation; biomaterial interface interaction; covalent bond; dental implants; extracellular matrix; immunologic assay /test; spectrometry; tissue /cell culture; titanium

The objective of this study is to develop a layered chemical surface on titanium implants that will promote the secretion of extracellular matrix on and the attachment of cells to the implant and that does not induce a foreign body reaction. An unoxidized layer of titanium will be deposited on cell culture dishes by vacuum evaporation or sputter-coating. A second layer will be self-assembling, quasi-crystalline alkane layer that will be attached to the titanium layer. This monolayer will furnish an exposed chemical group for the covalent bonding of a proteinaceous layer, such as basement membrane proteins, which will promote cell attachment. This assembly will be used as a culture surface to ascertain the biocompatibility of the surface. Future studies will examine the success of this assembly in vivo. The initial titanium film and the alkane layer will be analyzed using photoacoustic spectroscopy and Auger spectroscopy to ascertain the composition of the film. The presence and coverage of protein layer will be determined by specific staining, antibody techniques and spectrophotometry. The attachment of fibroblasts and isolated bone cells will be determined by serial washing studies and electron microscopic examination. Deposition of new proteins in the attachment region will be assayed by radiolabelling, autoradiography, and detection with specific antibodies. The development of a fully biocompatible surface for implants will promote the integration of implants into the biological systems. This development will lead to the production of prostheses that expoit normal biological processes and that induce little or no foreign body reaction. These results will have direct or indirect application in all fields of medicine that employ transition-metal prostheses.

这项研究的目的是在 钛植入物将促进细胞外基质的分泌 细胞附着在植入物上，不会诱导 异物反应。 一层未氧化的钛将沉积在 通过真空蒸发或溅射涂层进行细胞培养。 一秒钟 层将是自组装的，准晶体烷烃层将是 附着在钛层上。 这个单层将提供一个暴露的 化学基团用于蛋白质层共价键 地下膜蛋白将促进细胞附着。 这 组装将用作培养表面以确定生物相容性 的表面。 未来的研究将研究该集会在 体内。 最初的钛膜和烷烃层将使用 光声学光谱和螺旋体光谱法以确定 电影的组成。 蛋白质层的存在和覆盖范围将是 通过特定染色，抗体技术和分光光度法测定。 成纤维细胞和分离的骨细胞的附着将由 串行洗涤研究和电子显微镜检查。 沉积 附着区域中的新蛋白质将通过radiolabelling测定， 放射自显影和特定抗体检测。 的发展 植入物的完全生物相容的表面将促进 植入生物系统。 这种发展将导致 生产假体，这些假体会揭示正常的生物学过程 很少或没有异物反应。 这些结果将直接 或在所有采用的医学领域的间接应用 过渡金属假体。