Cell Isolation and Organ Function Core

细胞分离和器官功能核心

• 批准号: 10437830
• 负责人: Elizabeth O Harrington
• 金额: $ 32.49万
• 依托单位: OCEAN STATE RESEARCH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-20 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10437830
• 关键词: Acute Respiratory Distress Syndrome; Atherosclerosis; Biology; Blood Vessels; Bronchopulmonary Dysplasia; Businesses; Cardiac Myocytes; Cardiac development; Cardiopulmonary; Cell Separation; Cell physiology; Cells; Centers of Research Excellence; Congenital Heart Defects; Consultations; Cultured Cells; Data; Development; Disease Progression; Endothelial Cells; Endothelium; Ensure; Experimental Designs; Fibroblasts; Functional disorder; Goals; Heart; Heart Diseases; Image; Image Analysis; Injury; Interruption; Lasers; Lead; Lung; Lung diseases; Measurement; Mutation; Organ; Pathogenesis; Phase; Phenotype; Procedures; Process; Productivity; Pulmonary Hypertension; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; Rhode Island; Services; Structure; System; Technology; Tissues; Translating; Vascular Diseases; Vascular remodeling; angiogenesis; cell type; genetic manipulation; heart function; idiopathic pulmonary fibrosis; in vivo; injury and repair; instrumentation; morphometry; novel; pulmonary function; repaired; response; response to injury; skills; tool; transcription factor; vascular injury

The central goals of the CardioPulmonary Vascular Biology (CPVB) Center for Biomedical Research Excellence (COBRE) are to understand basic mechanisms of vascular disease pathogenesis and to translate these findings into treatments for heart and lung diseases. Vascular injury, repair, and remodeling are critical to the pathogenesis of the conditions studied in Phase II projects--bronchopulmonary dysplasia (BPD), atherosclerosis, idiopathic pulmonary fibrosis (IPF), pulmonary hypertension, and acute respiratory distress syndrome (ARDS). Cardiac development and remodeling in response to injury similarly include both adaptive and maladaptive responses. Dysfunction of angiogenesis, the reparative response of the endothelium to injury, and aberrant vascular remodeling have been proposed to occur in ARDS, BPD, IPF and other heart and lung conditions. In addition, some congenital heart defects result from mutations in transcription factors that regulate cardiomyocyte and endothelial cell (EC) function in development. Thus, an increased understanding of the mechanisms regulating the adaptation of the vasculature to injury and repair may lead to unique approaches to interrupt disease progression or to promote or enhance cardiopulmonary vascular repair. During Phase I the Cell/Organ Core developed centralized systems for cell isolations and organ function measurements and provided reproducible and accurate data for COBRE Projects. Additional services underway to enhance the Cell/Organ Core capabilities for the vascular biologists in Rhode Island and Phase II investigators are expansion of i) type of cell isolations; ii) immunohistochemical analyses; iii) EC function of primary isolates; iv) laser Doppler imaging; and v) in vivo angiogenesis. The Core will offer consultation on experimental design, provide cutting edge technological approaches, generate and analyze data, and serve as a resource for CPVB COBRE investigators and others in Rhode Island. The Core will also develop a business plan for financial stability of existing services and identify novel business or collaborative opportunities to ensure sustainability of the CPVB core. The overall goal of the Cell/Organ Core is to facilitate the scientific objectives and technical repertoire of the Project and Pilot Investigators by the following specific aims. (1) Enhance the productivity of CPVB COBRE research projects by offering reproducible, economical, and high quality i) cell isolation, propagation, phenotypic characterization and functional assessment ii) assessment of heart, lung, vessel structure and function and angiogenesis, and (iii) transient gene manipulation in vivo. (2) Acquire, establish, and disseminate technologies and instrumentation to provide state of the art research tools for vascular biology research in Rhode Island. (3) Establish processes and procedures that support the sustainability of the Cell/Organ Core. The CPVB COBRE Cell/Organ Core will support vascular biology research, including new investigators, projects, technical approaches and ideas that enhance the impact of research in Rhode Island.

心肺血管生物学 (CPVB) 生物医学研究中心的中心目标 卓越（COBRE）是了解血管疾病发病机制的基本机制并转化为 这些发现可用于治疗心脏和肺部疾病。血管损伤、修复和重塑至关重要 第二阶段项目研究的病症——支气管肺发育不良（BPD）的发病机制， 动脉粥样硬化、特发性肺纤维化 (IPF)、肺动脉高压和急性呼吸窘迫 综合征（ARDS）。响应损伤的心脏发育和重塑同样包括适应性 和适应不良反应。血管生成功能障碍，内皮对损伤的修复反应， 异常血管重塑已被认为发生在 ARDS、BPD、IPF 和其他心肺疾病中 状况。此外，一些先天性心脏病是由转录因子突变引起的，这些突变 调节发育过程中的心肌细胞和内皮细胞 (EC) 功能。从而加深了了解 调节脉管系统对损伤和修复的适应的机制可能会导致独特的 阻止疾病进展或促进或增强心肺血管修复的方法。 在第一阶段，细胞/器官核心开发了用于细胞分离和器官功能的集中系统 测量并为 COBRE 项目提供可重复且准确的数据。附加服务 正在进行中，旨在增强罗德岛州血管生物学家的细胞/器官核心能力和第二阶段 研究人员正在扩展 i) 细胞分离类型； ii) 免疫组织化学分析； iii) EC 功能 初级分离株； iv) 激光多普勒成像； v) 体内血管生成。核心将提供咨询 实验设计，提供前沿技术方法，生成和分析数据，并作为 为罗德岛州 CPVB COBRE 调查人员和其他人员提供的资源。核心还将发展一项业务 规划现有服务的财务稳定性，并确定新的业务或合作机会 确保 CPVB 核心的可持续性。细胞/器官核心的总体目标是促进科学 项目和试点研究人员通过以下具体目标确定其目标和技术手段。 (1) 通过提供可重复、经济且高效的方案，提高 CPVB COBRE 研究项目的生产力 质量 i) 细胞分离、增殖、表型表征和功能评估 ii) 评估 心脏、肺、血管结构和功能以及血管生成，以及（iii）体内瞬时基因操作。 (2) 获取、建立和传播技术和仪器以提供最先进的研究工具 用于罗德岛州的血管生物学研究。 (3) 建立支持的流程和程序 细胞/器官核心的可持续性。 CPVB COBRE 细胞/器官核心将支持血管生物学 研究，包括新的研究者、项目、技术方法和想法，以增强影响力 罗德岛州的研究。