Antimicrobial Resistant Bacteria: Exposures and Health of Cattle Workers

耐药细菌：养牛工人的接触和健康

• 批准号: 10437480
• 负责人: Sheryl Magzamen
• 金额: $ 59.42万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10437480
• 关键词: Antimicrobial; Resistant; Bacteria; Exposures; Health

Abstract Livestock workers are at the frontline of exposure to agriculture-generated bioaerosols containing a diverse mixture of respiratory pathogens. The misuse and overuse of antibiotics in agriculture has been posited as a potential driver in the accelerated development of antibiotic resistant genes (ARG). Animal handling, waste management, and bioaerosol generation may contribute to relatively high personal exposure to antibiotic resistant bacteria (ARB) on farms. The short- and long-term health effects of these bacteria are currently unknown. Moreover, workers may act as a vector – disseminating ARG and viruses. Specifically, cattle workers are at greater risk of Influenza D virus, an emerging pathogen associated with acute respiratory disease. The workers may also pose a threat to the animals, such as introducing strains of multi-drug resistant tuberculosis (TB). We propose to address critical questions that remain about the impacts of worker exposure to respiratory pathogens and transmission to animals, farms and the larger community. The eighteen year collaborative research program conducted by the High Plains Intermountain Center for Agricultural Health and Safety and regional dairy and beef producers offers a unique opportunity to investigate the role of workplace exposure on human-hosted bacterial communities (microbiome), associated antibiotic resistance genes (ARG, or resistome) and respiratory viruses (virome) and respiratory health in cattle workers. The research objective is to ascertain if ARG and viruses are underlying risk factors for subclinical markers of respiratory inflammation. The central hypothesis is that the nasal microbiome of cattle workers will have distinct microbial communities and genetic constitution that are associated with measurable levels of inflammation and airway resistance as compared to occupational controls and housemates. The specific aims are to: (1) compare and contrast the composition of the microbiome, resistome, and virome among dairy and beef workers; (2) model exposure-response relationships in the context of microbiome, resistome, virome and inflammatory markers and airway resistance among dairy and beef workers; and (3) ascertain subclinical health impacts of shared microbiome, resistome, and virome between occupational and household settings. This project unites scientists with expertise in occupational health and exposure science, epidemiology, microbiology, genetics, and bioinformatics. This study will contribute to greater awareness of workplace exposures and transmission of ARG and emerging viruses among dairy and beef workers.

抽象的 畜牧业工人处于接触农业产生的生物气溶胶的最前线，其中含有多种物质 农业中滥用和过度使用抗生素已被认为是呼吸道病原体的混合物。 抗生素抗性基因（ARG）加速发展的潜在驱动力。 管理和生物气溶胶的产生可能导致相对较高的个人接触抗生素 目前，这些细菌对农场的短期和长期健康影响。 此外，工人可能充当传播 ARG 和病毒的媒介。 感染 D 型流感病毒的风险更大，D 型流感病毒是一种与急性呼吸道疾病相关的新兴病原体。 工人还可能对动物构成威胁，例如引入多重耐药结核菌株 （结核病）。我们建议解决有关工人接触呼吸道的影响的关键问题。 病原体及其向动物、农场和更大社区的传播十八年合作。 高平原山间农业健康与安全中心进行的研究计划 区域乳制品和牛肉生产商提供了一个独特的机会来调查工作场所接触对健康的影响 人类宿主细菌群落（微生物组）、相关抗生素抗性基因（ARG 或抗性组） 呼吸道病毒（病毒组）和养牛工人的呼吸道健康。 ARG 和病毒是否是呼吸道炎症亚临床标志物的潜在危险因素。 假设养牛工人的鼻腔微生物组将具有独特的微生物群落和遗传 与可测量的炎症和气道阻力水平相关的体质 职业控制和室友的具体目的是：（1）比较和对比的组成。 乳制品和牛肉工人的微生物组、耐药组和病毒组 (2) 模型暴露反应； 微生物组、耐药组、病毒组和炎症标志物以及气道阻力的关系 在乳制品和牛肉工人中；(3) 确定共享微生物组、耐药组、 该项目将具有专业知识的科学家联合起来。 本研究涉及职业健康和暴露科学、流行病学、微生物学、遗传学和生物信息学。 将有助于提高人们对工作场所 ARG 和新出现病毒的暴露和传播的认识 乳制品和牛肉工人中。