Automated Multiscale Super-resolution Confocal Microscope

自动化多尺度超分辨率共焦显微镜

• 批准号: 10431707
• 负责人: Bill William Kiosses
• 金额: $ 51.44万
• 依托单位: LA JOLLA INSTITUTE FOR IMMUNOLOGY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2023-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431707
• 关键词: 2019-nCoV; Aging; Atherosclerosis; Biological Process; Biology; Cells; Computer software; Core Facility; Disease; Funding; Growth; Histology; Image; Immune; Immunology; Inflammation; Institutes; Insulin-Dependent Diabetes Mellitus; Investigation; Journals; Malignant Neoplasms; Microscope; Microscopy; Molecular; Mus; NIH Grants and Contracts; Nature; Pathway interactions; Principal Investigator; Proteins; Publishing; Research; Research Institute; Research Personnel; Research Project Grants; Resolution; Resources; Science; Scientist; Signal Transduction; Speed; System; United States National Institutes of Health; Viral; behavioral study; cell type; detector; human tissue; imaging capabilities; innovation; instrument; instrumentation; novel

ABSTRACT The La Jolla Institute for Immunology (LJI) is a premier immunology research institute in the US whose 20 principal investigators are supported by ~$39 million in NIH grants and contracts. At LJI, we study the behavior of immune cells in diseases prominent in the US and around the world, including viral biology, type 1 diabetes, atherosclerosis, cancer, and inflammation. Nearly all of our scientists use imaging to visualize cellular interactions, signaling, and function with results published in Nature, Science, and other prominent journals. However, as our research aims evolve, and to progress further in the research projects listed in this application, we need a reliable and versatile super-resolution confocal microscope with multiscale imaging capability. The LJI Microscopy and Histology Core Facility is a dynamic, scientifically driven, multi-user resource with a remarkable potential for growth. The instrumentation in the LJI Microscopy Core Facility includes an aging Zeiss LSM 780 that is used by many NIH funded projects, with aims ranging from the investigation of matrix protein assembly in SARS-CoV-2, novel cell types in atherosclerosis, molecular pathways leading to Type 1 diabetes, and many others. This system is nearing the end of its useful lifetime and a replacement microscope is needed. Here, we seek support for purchasing an automated, state-of-the-art, super-resolution microscope that allows for multiscale, high-throughput investigation of mouse and human tissues. Dynamic biological processes can be probed thanks to innovative software, highly sensitive detectors, and unparalleled acquisition speed. After comparing instruments on the market from all major companies, it is clear that the Zeiss LSM 980 with Airyscan 2 system is the best suited for the needs of LJI investigators and collaborators, and if funded, it will undoubtedly have a significant impact on NIH-supported scientific progress.

抽象的 拉霍亚免疫学研究所 (LJI) 是美国首屈一指的免疫学研究所， 20 名主要研究人员获得约 3900 万美元的 NIH 拨款和合同支持。在 LJI，我们研究 美国和世界各地常见疾病中免疫细胞的行为，包括 1 型病毒生物学 糖尿病、动脉粥样硬化、癌症和炎症。几乎我们所有的科学家都使用成像来可视化细胞 相互作用、信号传导和功能，其结果发表在《自然》、《科学》和其他著名期刊上。 然而，随着我们研究目标的发展，以及本申请中列出的研究项目的进一步进展， 我们需要一种可靠、多功能、具有多尺度成像能力的超分辨率共焦显微镜。这 LJI 显微镜和组织学核心设施是一个动态的、科学驱动的、多用户资源，具有 显着的增长潜力。 LJI 显微镜核心设施中的仪器包括老化的蔡司 许多 NIH 资助的项目都使用 LSM 780，其目的包括基质蛋白的研究 SARS-CoV-2 中的组装、动脉粥样硬化中的新细胞类型、导致 1 型糖尿病的分子途径、 以及许多其他人。该系统的使用寿命已接近尾声，需要更换显微镜。 在此，我们寻求支持购买一台自动化、最先进的超分辨率显微镜， 允许对小鼠和人体组织进行多尺度、高通量研究。动态生物 借助创新的软件、高灵敏度探测器和无与伦比的技术，可以探测过程 采集速度。在比较了市场上各大公司的仪器后，很明显 配备 Airyscan 2 系统的 Zeiss LSM 980 最适合 LJI 研究人员和合作者的需求， 如果获得资助，无疑将对 NIH 支持的科学进步产生重大影响。