High-resolution ex vivo microCT for biomedical imaging

用于生物医学成像的高分辨率离体 microCT

• 批准号: 10426416
• 负责人: Ali Murat Maga
• 金额: $ 41.33万
• 依托单位: SEATTLE CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10426416
• 关键词: 14 year old; 3-Dimensional; Affect; Area; Back; Basic Science; Biology; Child; Communities; Computer software; Contract Services; DNA Sequence Alteration; Data; Data Storage and Retrieval; Development; Devices; Dimensions; Equipment; Fred Hutchinson Cancer Research Center; Funding; Goals; Grant; Human; Human Resources; Image; Image Analysis; Injury; Institutes; Institution; Maintenance; Manuals; Microscopic; Minor; Organ; Pathology; Phenotype; Preparation; Productivity; Research; Research Institute; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Specimen; Syndrome; System; Systems Biology; Three-Dimensional Image; Time; Tissue imaging; United States National Institutes of Health; Universities; Washington; Whole Organism; Work; base; biomedical imaging; bone; data acquisition; end of life; imaging system; in vivo; innovation; instrument; microCT; microscopic imaging; new technology; tissue regeneration

Project Summary The goal of this application is to replace an essential, 14 year old in-vivo microCT imaging system at the Seattle Children’s Research Institute (SCRI) with a state-of-the-art Bruker Skyscan 1272 high-resolution 3D ex- vivo microCT imaging system. This instrument is required to conduct a diversity of research projects, which require imaging both at large-scale (whole organisms/organs) and microscopic-scale. This imaging resource will support the activities of 11 major and 4 minor users focused on three cross-cutting research themes: (1) Genetic mutations underlying human congenital syndromes that affect multiple organs, (2) development and pathology of vasculature, (3) mechanisms of tissue regeneration after ischemic damage or injury, and (4) basic research in bone biology and remodeling. A portfolio of 18 funded and 4 pending NIH grants, as well as funding from other federal agencies like NSF currently supports these projects. This exciting new technology allow users to rake benefits from the technological improvements introduced to microCT imaging and sample preparation in the last decade. As shown in our pilot data, with the new ex-vivo system we will be able to image tissues with higher resolution, with better image quality and clarity compared to our current end-of-life instrument. Further, some of the listed projects involve tedious phenotypic screens, which have been hampered by the manual sample navigation and data acquisition with our existing system. With an automated sample changer and a control software that automatically determines the optimal settings, based specimen dimensions, the proposed equipment will help our users overcome these problems, and achieve higher analytical throughput, while obtaining better quality data. There is strong Institutional commitment for the instrument, including state-of-the-art imaging space, coverage of service contracts, maintenance personnel effort in at least the first 5 years, backed-up data storage, and 3-D image analysis resources. While SCRI and University of Washington investigators comprise the main user group, these instruments would be accessible to other investigators from allied institutions such as the Fred Hutchinson Cancer Research Center, Institutes for Systems Biology and others, creating a unique and valuable community resource for macroscopic-to- microscopic imaging. In addition, there is extensive imaging expertise present at the SCRI Center that will house and support the device (PI, Maga, Co-I, Shih and Yu). This will guide the proper use, time allocation and maintenance of the instrument. This imaging resource would immediately influence the productivity and innovation of ongoing work and will be critical in furthering the long-range objectives of the biomedical community in the greater Seattle area.

项目概要 该应用的目标是取代已有 14 年历史的重要体内 microCT 成像系统 西雅图儿童研究所 (SCRI) 拥有最先进的 Bruker Skyscan 1272 高分辨率 3D ex- 开展多种研究项目需要该仪器。 需要大规模（整个生物体/器官）和微观规模的成像此成像资源。 将支持 11 个主要用户和 4 个次要用户的活动，重点关注三个交叉研究主题：(1) 影响多个器官的人类先天性综合症的基因突变，（2）发育和 脉管系统的病理学，（3）缺血性损伤或损伤后组织再生的机制，以及（4）基础 骨生物学和重塑方面的研究，包括 18 项资助和 4 项待决的 NIH 资助以及 NIH 资助。 目前，这项令人兴奋的新技术得到了 NSF 等其他联邦机构的资助。 让用户能够从 microCT 成像和样本的技术改进中获益 正如我们的试点数据所示，通过新的离体系统，我们将能够成像。 与我们当前的报废组织相比，具有更高分辨率、更好的图像质量和清晰度 此外，一些列出的项目涉及繁琐的表型筛选，这些项目已被 我们现有系统的手动样本导航和数据采集受到了阻碍。 进样器和控制软件可根据样本自动确定最佳设置 尺寸，所提出的设备将帮助我们的用户克服这些问题，并实现更高的 分析吞吐量，同时获得更高质量的数据有强有力的机构承诺。 仪器，包括最先进的成像空间、服务合同范围、维护人员 至少前 5 年的努力、备份数据存储和 3D 图像分析资源。 华盛顿大学的研究人员构成主要用户群体，这些工具将可供使用 来自弗雷德·哈钦森癌症研究中心、研究所等联盟机构的其他研究人员 对于系统生物学和其他领域，为宏观到-创建独特且有价值的社区资源 此外，SCRI 中心还拥有丰富的成像专业知识。 安置并支持设备（PI、Maga、Co-I、Shih 和 Yu），这将指导正确使用、时间分配和维护。 该成像资源将立即影响仪器的生产率和维护。 正在进行的工作的创新，对于推进生物医学的长期目标至关重要 大西雅图地区的社区。