Bioluminescence and Fluorescence Optical Animal Imager

生物发光和荧光光学动物成像仪

• 批准号: 10419960
• 负责人: GUILLERMO MARQUES
• 金额: $ 44.73万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10419960
• 关键词: 18 year old; 3-Dimensional; Affect; Animals; Autoimmunity; Bioluminescence; Cells; Detection; Drug Delivery Systems; Fluorescence; Funding; HIV; Image; Immune system; Immunotherapy; Ischemic Stroke; Laboratories; Mammary Neoplasms; Mus; Muscular Dystrophies; Optics; Physiological; Prostate Cancer therapy; Research; Research Personnel; Research Project Grants; Sampling; Systems Development; Time; United States National Institutes of Health; Vendor; antitumor drug; bioluminescence imaging; cancer therapy; flexibility; fluorescence imaging; imager; imaging capabilities; in vivo imaging system; instrument; multimodality; optical imager

Project Summary/Abstract The Perkin Elmer IVIS Spectrum requested is a whole animal optical imager capable of 2D and 3D fluorescence and bioluminescence imaging. The instrument provides high throughput (up to 10 mice at a time), high sensitivity (down to single cell detection), and a physiological platform that keeps the animals anesthetized and warm during imaging. Like other IVIS instruments, the IVIS Spectrum is calibrated in absolute radiance units, allowing comparison between samples imaged in different instruments. This instrument will replace an 18-year-old optical animal imager that is no longer supported by the vendor. More than 32 laboratories depend on the current instrument, and their research would be catastrophically affected if the instrument failed. Among those laboratories are the 10 NIH- funded investigators that are the users in this proposal. Being able to provide these investigators with the Perkin Elmer IVIS Spectrum animal imager would protect their current capabilities of bioluminescence and restore the fluorescence imaging capabilities available in the past. In addition the new instrument has better sensitivity and multimodal flexibility. The research projects thus enhanced will cover studies of immune system development and autoimmunity, surveillance of HIV CNS reservoirs, immune therapy of cancer, anti-tumor drug delivery in mammary and prostate cancer, and therapies for ischemic stroke and Duschenne’s muscular dystrophy.

项目概要/摘要 所需的 Perkin Elmer IVIS Spectrum 是一款能够进行 2D 成像的整体动物光学成像仪 该仪器提供高3D荧光和生物发光成像。 吞吐量（一次最多 10 只小鼠）、高灵敏度（低至单细胞检测）和 生理平台，使动物在成像过程中保持麻醉和温暖。 与其他 IVIS 仪器一样，IVIS Spectrum 以绝对辐射度单位进行校准，允许 该仪器将在不同仪器中成像的样品之间进行比较。 更换已使用 18 年、供应商不再支持的光学动物成像仪。 超过 32 个实验室依赖于当前的仪器，他们的研究将 如果仪器出现故障，这些实验室将受到灾难性的影响，其中包括 10 个 NIH-实验室。 资助的研究人员是本提案中能够提供这些的用户。 研究人员使用 Perkin Elmer IVIS Spectrum 动物成像仪来保护他们的 当前生物发光能力并恢复荧光成像能力 此外，新仪器具有更好的灵敏度和多模式。 由此增强的研究项目将涵盖免疫系统的研究。 发育和自身免疫、HIV CNS 储存库的监测、免疫治疗 癌症、乳腺癌和前列腺癌的抗肿瘤药物输送以及治疗 缺血性中风和杜氏肌营养不良症。