DIAGNOSTIC AND LABORATORY METHODS FOR ACYCLOVIR-RESISTANT HERPES SIMPLEX VIRUS

耐阿昔洛韦单纯疱疹病毒的诊断和实验室方法

• 批准号: 3746932
• 负责人: SHARON SAFRIN
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3746932
• 关键词: AIDS therapy; HIV infections; Herpes simplex disease; acyclovir; antiviral agents; autoradiography; clinical trials; diagnostic tests; dosage; drug resistance; dyes; enzyme inhibitors; foscarnet; herpes simplex virus 1; herpes simplex virus 2; human subject; hydrazones; nucleic acid hybridization; opportunistic infections; pharmacokinetics; pyrimidines; virus replication

Along with reports of the increasing prevalence of antiviral drug resistance among HIV, cytomegalovirus, varicella-zoster virus and influenza isolates there has been an alarming increase in clinically-significant infections due to acyclovir-resistant herpes simplex virus (HSV) in patients with HIV infection. Although the results of susceptibility testing are invariably used to guide the chemotherapeutic management of these patients, little systematic information is available to the clinician regarding the interpretation of results of these tests, as the testing methods and susceptibility breakpoints are neither standardized nor based on extensive clinical correlations. We propose to rationalize antiviral susceptibility testing of HSV by: 1. Comparing the methods currently used to test HSV for susceptibility to antivirals, 2. Correlating the results of susceptibility testing with the outcome of chemotherapy, and 3. Prospectively evaluating the test criteria in clinical trials of new and existing antivirals. The results of these studies will place susceptibility testing of HSV on the same rational footing as testing of bacteria for susceptibility to antibacterial agents.

以及抗病毒药物患病率增加的报道 艾滋病毒，巨细胞病毒，水痘带状疱疹病毒和流感的耐药性 隔离株临床上有很大的意义 由于抗阳性抗体的疱疹病毒（HSV）引起的感染 HIV感染患者。 虽然易感性的结果 测试总是用于指导化学治疗管理 这些患者，临床医生几乎没有系统信息 关于这些测试结果的解释，作为测试 方法和敏感性断点既不是标准化也不是基于标准化的 广泛的临床相关性。 我们建议合理化抗病毒 HSV的敏感性测试：1。比较当前使用的方法 为了测试HSV的抗病毒药易感性，2。 通过化学疗法的结果进行敏感性测试和3。 在新的和 现有的抗病毒药。 这些研究的结果将放置 HSV在与测试相同的合理基础上对HSV的敏感性测试 细菌对抗菌剂的敏感性。