MAPPING THE HUMAN Y CHROMOSOME

绘制人类 Y 染色体图谱

• 批准号: 2208676
• 负责人: David C. Page
• 金额: $ 40.83万
• 依托单位: WHITEHEAD INSTITUTE FOR BIOMEDICAL RES
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2208676
• 关键词: chromosome deletion; chromosome disorders; complementary DNA; cytogenetics; genetic mapping; human genetic material tag; human subject; male; molecular cloning; mutant; nucleic acid sequence; oligonucleotides; sequence tagged sites; sex chromosomes

This project focuses on the human Y chromosome in the context of the Human Genome Project. The principal long-term goal of this project is complete definition of the genetic content of the human Y chromosome. Toward that end, overlapping recombinant DNA clones spanning the chromosome will be isolated, and these clones together with landmarks based in the DNA sequence itself will be used to construct a high- resolution physical map of the chromosome. This physical map should provide a foundation for future efforts to determine the DNA sequence of the Y chromosome in its entirety. In parallel, a second map of the chromosome will be constructed using naturally occurring deletions of the Y chromosome as found in human individuals. The resulting information will provide a framework for elucidating the function of the Y chromosome in medically relevant processes as diverse as spermatogenesis, tumorigenesis, and embryonic development.

该项目的重点是在人类y染色体上 人类基因组项目。该项目的主要长期目标是 人类Y染色体的遗传含量的完整定义。 为此，重叠的重组DNA克隆跨越了 染色体将被隔离，这些克隆与地标在一起 基于DNA序列本身将用于构建高 染色体的分辨率物理图。这个物理地图应该 为确定DNA序列的未来努力提供了基础 Y染色体的整体。同时，第二张地图 染色体将使用天然存在的删除 在人体中发现的Y染色体。结果 信息将为阐明功能提供一个框架 在医学相关过程中，Y染色体如多样化 精子发生，肿瘤发生和胚胎发育。