GENETIC MAPPING AND DNA STRUCTURE OF HUMAN CHROMOSOME 11

人类 11 号染色体的基因图谱和 DNA 结构

• 批准号: 2208697
• 负责人: David Housman
• 金额: $ 46.78万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1996-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2208697
• 关键词: DNA; artificial chromosomes; chromosome translocation; chromosomes; clone cells; cytogenetics; gel electrophoresis; gene expression; gene mutation; genetic mapping; genetic markers; genetic recombination; human genetic material tag; human population genetics; human tissue; in situ hybridization; linkage mapping; molecular cloning; molecular pathology; nucleic acid hybridization; nucleic acid sequence; nucleic acid structure; polymerase chain reaction; tissue /cell culture

During the next granting period, our goals will focus on the utilization of the cloned genomic DNA sequences in YAC contigs from the short arm of chromosome 11 to develop a detailed map of expressed genes for this chromosome arm. In order to carry out this objective our specific goals will be as follows: 1. We will complete YAC contig generation for the short arm of chromosome 11. To achieve this goal we will continue to implement the alu pcr based filter hybridization strategy we have devised to achieve a high level of efficiency and economy in YAC contig building. 2. We will characterize the genomic DNA isolated by the YAC cloning to verify the accuracy with which genomic DNA sequences from human DNA are represented in the YACs 3. We will utilize DNA from 11p YAC contigs to identify sequences of expressed genes in the genomic DNA of this chromosome arm. To achieve this objective efficiently we will continue to implement the exon amplification methodology we developed during the previous granting period. 4. We will isolate cDNAs for each expressed gene we identify. To facilitate this goal we will implement pcr based cDNA library screening strategies to maximize efficiency of library screening. 5. We will attempt to develop and implement an efficient strategy for obtaining full length cDNA coverage for each gene. 6. We will carry out sequence analysis of cDNAs and corresponding segments of genomic DNA. 7. We will develop YAC contigs in the mouse for the genomic regions homologous to the 11p regions on which we have focused. We will utilize these YAC contigs in order to develop a comparative map of expressed genes for the syntenic chromosome segments between the two species.

在下一个授予期内，我们的目标将重点放在利用率上 YAC重叠群中的克隆基因组DNA序列的短臂的短臂 11染色体为此开发详细的表达基因图 染色体臂。 为了实现这一目标，我们的具体目标 如下： 1。我们将完成染色体短臂的YAC重叠生成 11。为了实现这一目标，我们将继续实施基于ALU PCR的 过滤杂交策略，我们已经设计了以达到高水平的 YAC重叠建筑中的效率和经济。 2。我们将表征YAC克隆分离的基因组DNA 验证人类DNA的基因组DNA序列的准确性 在YAC中代表 3。我们将利用11p YAC重叠群的DNA来识别 在该染色体组的基因组DNA中表达基因。 实现 这个目标有效地我们将继续实施外显子 我们在上一个授予期间开发的放大方法 时期。 4。我们将为我们识别的每个表达基因分离cDNA。 到 促进这个目标，我们将实施基于PCR的cDNA库筛选 最大化图书馆筛选效率的策略。 5。我们将尝试制定和实施有效的策略 获得每个基因的全长cDNA覆盖率。 6。我们将进行cDNA和相应的序列分析 基因组DNA的片段。 7。我们将在小鼠中为基因组区域开发YAC重叠群 与我们关注的11p区域同源。 我们将利用 这些YAC重叠群是为了开发表达的比较图 两种物种之间同义染色体片段的基因。