CORE--RADIOIMMUNOASSAY

核心--放射免疫分析

• 批准号: 5212843
• 负责人: RICHARD G ALLEN
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5212843
• 关键词: adrenocorticotropic hormone; atrial natriuretic peptide; biomedical facility; endorphins; genetically modified animals; high performance liquid chromatography; hypothalamic pituitary axis; laboratory mouse; oxytocin; pituitary adrenal axis; proopiomelanocortin; radioimmunoassay

The goal of this core facility is to provide the technical support for the performance of the variety of RIAs required by the individual projects of the Program. The rationale for establishing this core is multifold. First, we will centralize most of the RIAs in one laboratory. Second, we will not duplicate supplies and efforts. Third, the data generated by this core facility will foster scientific exchange between individual laboratories. Lastly, the core director has over fifteen years of experience in developing and using RIAs specific for POMC-derive peptides, as well as many other peptide hormones. Virtually all of the studies within measure the POMC-derived peptides, ACTH, beta-endorphin, the alpha and beta-melanocyte stimulating hormones (CLIP) corticotropin- like-intermediate-lobe-peptide, as well as assist Project VI assaying CRH, SST, ANP, AVP and oxytocin.

该核心设施的目标是为 个人需要的各种RIA的表现 该计划的项目。 建立此核心的理由是 多重。 首先，我们将把大部分RIA集中在一个实验室中。 其次，我们不会重复供应和努力。 第三，数据 该核心设施产生的将促进科学交流 个别实验室。 最后，核心导演有15多个 多年在开发和使用针对POMC-derive的RIA的经验 肽以及许多其他肽激素。 几乎所有 在测量POMC衍生的肽ACTH，β-内啡肽的研究中， α和β-甲状腺细胞刺激激素（夹）皮质激素 - 例如中间叶肽，并协助项目VI分析 CRH，SST，ANP，AVP和催产素。