CORE--ANIMAL RESOURCES

核心——动物资源

• 批准号: 5212699
• 负责人: ANN MARIE LAWLER
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5212699
• 关键词: Downs syndrome; animal breeding; animal colony; animal population genetics; biomedical facility; chromosome 21; clone cells; cryopreservation; disease /disorder model; embryo /fetus tissue /cell culture; embryonic stem cell; genetic strain; genetically modified animals; laboratory mouse; microinjections; polymerase chain reaction; southern blotting

The goal of the Animal Resources core will be to generate and maintain transgenic mouse lines derived from genetically manipulated embryonic stem (ES) cells lines that will be developed in Projects II and III. The core will also provide animals for analysis, testing, and mating as needed by Program II, III, IV and Core B. Primarily, mouse lines will be generated through blastocyst injection of ES cells, however, this core will also be able to generate standard transgenic mouse lines through pronuclear microinjection of DNA constructs, as necessary. It is estimated that two to three ES cell lines will be injected for each construct and that one to two lines of mice will be maintained for each construct, depending on the phenotype and expression levels in each line. The Core will maintain breeding stocks and study animals for six transgenic mouse lines and appropriate control strains that are currently under study in Program II and III and Core B. The Core will also provide animals for mating by Project IV. The Core will oversee DNA typing, breeding record, and pedigrees for these lies and all subsequent lines that are developed by the project. The Animals Core will also maintain breeding stocks of C57BL/6J mice for production of blastocysts and CD-1 females and vasectomized males for production of pseudopregnant host females. Breeding stocks of FVB/NJ mice and a transgenic line of FVB/NJ mice carrying a pSV40tkneo construct will be maintained to provide embryonic fibroblast feeder cells for maintenance and selection of ES cells. The average daily census for the Animal Core is expected to be 500. All animals will be maintained in micro-isolator cages in a pathogen-free facility at the Johns Hopkins Medical School. The Animal Core w ill also be able to maintain frozen stocks of embryos from transgenic lines that are no longer needed for immediate production of study animals.

动物资源核心的目标是生成和维护 源自遗传操纵的胚胎的转基因小鼠系 在项目II和III中将开发的茎（ES）细胞系。 这 核心还将为分析，测试和交配提供动物 程序II，III，IV和CoreB都需要 但是，可以通过胚泡注射ES细胞产生，但是该核心 还将能够通过 必要时对DNA构建体的前核显微注射。 这是 估计将注入两到三个ES细胞系 构造，每只小鼠将保持一到两行 构造，取决于每行的表型和表达水平。 核心将维持繁殖库存并研究六个 当前是转基因鼠标线和适当的控制菌株 在计划II和III和核心B中的研究中。核心也将提供 通过项目IV交配的动物。 核心将监督DNA键入， 这些谎言和所有后续行的繁殖记录和血统 该项目开发的。 动物核心也将维持 C57BL/6J小鼠的繁殖库存用于生产胚泡和CD-1 雌性和替代雄性生产假宿主的雄性 女性。 FVB/NJ小鼠的繁殖库存和FVB/NJ的转基因线 携带PSV40TKNEO构造的小鼠将维护以提供 用于维持和选择ES的胚胎成纤维细胞馈线细胞 细胞。 预计动物核心的平均每日人口普查为 500。所有动物都将在微隔离笼中维持 约翰·霍普金斯医学院的无病原体设施。 动物 核心还可以从中维持冷冻的胚胎库存 不再需要立即生产的转基因线 研究动物。