Rapidly Adaptable and Mass-Producible Microscopic Chiplets for Minimally-Instrumented Respiratory Viral Screening

用于微仪器呼吸道病毒筛查的快速适应性和可大规模生产的显微芯片

基本信息

批准号：
10348469
负责人：
Matthew Frederick Campbell
金额：
$ 11.43万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-10 至 2027-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10348469
关键词：
2019-nCoV Affect Algorithms Antibodies Antigens Applied Research Area Award Biological Biological Assay Biology Biomedical Engineering Biomedical Research Biosensor COVID-19 COVID-19 detection COVID-19 pandemic Cellular Phone Chemicals Clinical Containment Data Databases Development Development Plans Diagnostic Disease Disease Outbreaks Doctor of Medicine Economic Burden Electric Conductivity Engineering Environment Epidemic Eye Funding Future Goals Human Immobilization Immunoassay Individual Influenza Investigation K-Series Research Career Programs Label Light Measurement Medical Mentors Mentorship Methods Microfabrication Microscopic Middle East Respiratory Syndrome Coronavirus Mucous body substance Nucleic Acids Optics Partner in relationship Patients Pennsylvania Persons Plants Play Population Positioning Attribute Procedures Production Property Reader Research Research Personnel Resources Respiratory Tract Infections SARS coronavirus Sampling Scheme Schools Secure Semiconductors Silicon Solvents Source Specificity Specimen Surface Swab System Test Result Testing Thinness Time Training Transistors United States United States National Institutes of Health Universities Viral Viral Respiratory Tract Infection Virus Work antigen binding aptamer base career career development clinically relevant cohesion combat contagion design detector diagnostic platform diagnostic screening experience feasibility testing flexibility handheld equipment human disease instrument integrated circuit multiplex detection nanofabrication nanowire novel pandemic disease point of care portability professor receptor respiratory response saliva sample screening seasonal influenza sensor signal processing skills tenure track tool two-dimensional wireless

项目摘要

Project Summary Objective: Respiratory viral infections affect millions of individuals each year. Conducting frequent widespread viral screening tests can curb outbreaks by quickly identifying infectious persons. Unfortunately, no screening diagnostic platform exists with the capacity to test hundreds of millions of people daily during a pandemic. This proposal is the first step in developing a novel viral screening test to fill this gap. The assay will consist of microscopic circuits containing field effect transistors with antigen-specific receptors that are sensitive to particular viruses. These chiplets – barely visible to the human eye – will be powered by light and will transmit data using a light emitting diode, such that a few can be mixed into an extracted sample and illuminated with a handheld device to yield immediate results. This diagnostic will be scalable and inexpensive; millions of tiny chiplets can be fabricated simultaneously. It will be minimally instrumented; an ordinary cellphone with a strobe flash and camera will interface with the chips. It will be flexible, rapidly adaptable, and multiplexable; receptors specific to different or emerging viruses could be immobilized on distinct circuits, allowing multiple diseases to be detected simultaneously in a single patient sample. This diagnostic will thus be unmatched as a mass producible, simple to use, adaptable, and high throughput tool for frequent and widespread virus screening. Specific aims: The proposed diagnostic will be developed by pursuing the following specific aims. 1. Integrate biological field effect transistors into the existing optical wireless integrated circuit platform. 2. Develop a multiplexed detection scheme for interacting with optical wireless integrated circuits. 3. Demonstrate the test’s feasibility in a clinically relevant quantitative range using mock clinical specimens. Career development plan and career goals: Dr. Matthew Campbell (Ph.D., P.E.) is a postdoctoral researcher in the School of Engineering and Applied Science at the University of Pennsylvania, where his work is focused on fabricating microelectromechanical systems. The proposed K25 career development award will apply his nanofabrication skills toward biosensor development and extend his training and exposure into two new domains: (1) biomedical experimentation, and (2) medical biology. This proposal contains a cohesive mentorship and didactic strategy centered on these areas to accelerate his trajectory toward research independence. Completion of this multifaceted training plan will position Dr. Campbell with the cross-disciplinary skills and expertise necessary to become a leading investigator in the field of biomedical sensing diagnostics. Mentors and environment: Dr. Campbell is enthusiastically supported by the university and his strong mentoring team. His primary mentor is an expert in micromanufacturing (Prof. Igor Bargatin (Ph.D.)), and his co- mentors bring extensive experience in microscopic circuits (Prof. Marc Miskin (Ph.D.)), field effect transistor sensors (Prof. Charlie Johnson (Ph.D.) and Prof. Haim Bau (Ph.D.)), and viral respiratory tract infections (Prof. Ronald Collman (M.D.). This group will provide the ideal training situation for Dr. Campbell to develop this assay.

项目概要目的：呼吸道病毒感染每年影响数百万人。广泛的病毒筛查测试可以通过快速识别感染者来遏制疫情，但不幸的是，事实并非如此。筛查诊断平台能够每天对数亿人进行检测该提案是开发新型病毒筛查测试以填补这一空白的第一步。由含有场效应晶体管的微观电路组成，场效应晶体管具有敏感的抗原特异性受体这些小芯片——人眼几乎看不见——将由光驱动并传播。使用发光二极管获取数据，这样可以将一些数据混合到提取的样本中并用发光二极管照明这种诊断方法可扩展且价格低廉；可以同时制造小芯片；它将是一个带有闪光灯的普通手机。闪光灯和相机将与芯片连接，具有灵活、快速适应性和可复用性；针对不同或新出现的病毒的特异性可以固定在不同的电路上，从而允许多种疾病在单个患者样本中同时检测到该诊断结果将是无与伦比的。可生产、使用简单、适应性强、高通量的工具，用于频繁和广泛的病毒筛查。具体目标：建议的诊断将通过追求以下具体目标来制定。 1、将生物场效应晶体管集成到现有的光无线集成电路平台中。 2. 开发与光学无线集成电路交互的多路复用检测方案。 3. 使用模拟临床标本证明测试在临床相关定量范围内的可行性。职业发展规划和职业目标：Matthew Campbell博士（Ph.D., P.E.），博士后宾夕法尼亚大学工程与应用科学学院研究员，他的工作地点拟议的 K25 职业发展奖专注于制造微机电系统。将他的纳米制造技能应用于生物传感器开发，并将他的培训和接触扩展到两个方面新领域：（1）生物医学实验，（2）医学生物学。以这些领域为中心的指导和教学策略，以加速他的研究轨迹完成这项多方面的培训计划将使坎贝尔博士具备跨学科的能力。成为生物医学传感诊断领域的领先研究人员所需的技能和专业知识。导师和环境：坎贝尔博士得到了大学的热情支持和他强大的影响力。他的主要导师是微制造专家（Igor Bargatin 教授（博士）），他的合作伙伴是导师带来微观电路（Marc Miskin 教授（博士））、场效应晶体管方面的丰富经验传感器（Charlie Johnson 教授（博士）和 Haim Bau 教授（博士））和病毒性呼吸道感染（Prof. Ronald Collman（医学博士）。该小组将为 Campbell 博士开发该检测提供理想的培训环境。