Metabolic Phenotyping Core

代谢表型核心

基本信息

批准号：
10339349
负责人：
TIMOTHY M GRIFFIN
金额：
$ 27.97万
依托单位：
OKLAHOMA MEDICAL RESEARCH FOUNDATION
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-02-05 至 2026-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10339349
关键词：
Address Advisory Committees Aging Area Autoimmune Diseases Bioinformatics Biological Biological Assay Cardiovascular Diseases Cell Respiration Cells Cellular Metabolic Process Centers of Research Excellence Clinical Trials Complex Consult Consultations Data Data Analyses Decision Making Degenerative polyarthritis Development Diet Disease Doctor of Philosophy Ensure Environment Equipment Evaluation Experimental Designs Fostering Functional disorder Funding Future Genus Hippocampus Goals Growth Interdisciplinary Study Isotopes Leadership Malignant Neoplasms Mass Fragmentography Measurement Mediating Mentors Mentorship Metabolic Metabolism Metformin Methodology Methods Mitochondria Modeling Mus Obese Mice Oklahoma Pathology Phase Physiology Positioning Attribute Procedures Process Regulation Research Research Design Research Personnel Research Support Resources Role Services Standardization Structure Technical Expertise Techniques Time Tissues Tracer Training analytical method base comparative design exercise intervention experience follow-up in vivo instrument instrumentation interest meetings member metabolic abnormality assessment metabolic phenotype mouse development multidisciplinary new technology novel operation programs protein degradation skills stable isotope sustainable resource

项目摘要

The goal of the Center for Cellular Metabolism Research in Oklahoma (CMRO) Phase I COBRE proposal is to cultivate novel multidisciplinary research focusing on metabolism. As part of this overall goal, we created the Metabolic Phenotyping Core to facilitate the transition of our COBRE investigators to independent R01 funding and to develop metabolic research in Oklahoma. The projects proposed by the COBRE investigators seek to understand the mechanisms by which cellular metabolic processes drive or result from cardiovascular disease, autoimmune disease, and cancer. Because cellular metabolism is complex and poses many biological and technical challenges, the Core will provide a team of highly-trained directors and PhD-level scientific staff, access to state-of-the-art instruments that are carefully maintained, and mentored training in experimental design, implementation, and interpretation of metabolic data. The Core addresses three critical needs to support our COBRE investigators: 1) assessment of cellular respiration and mitochondrial function, 2) isotopic metabolic tracer analyses, and 3) metabolite-based metabolic profiling and bioinformatic analyses. To meet these needs, our specific aims are to: 1) provide expertise with study design, assay optimization, data analysis, and interpretation of results, as well as evaluate and develop new methodologies for in vivo metabolic phenotyping, and 2) provide COBRE investigators access to centralized equipment and appropriate scientific and technical training to facilitate the development of new expertise in cellular metabolic assessment. To support the COBRE Investigators, the Core will provide instrumentation for cellular respiration and mitochondrial function (e.g. Seahorse, Oroboros, and EPR), metabolite tracer analysis (LC-MS/MS), and metabolic profiling (GC-MS and GC-MS/MS). The Core will be directed by Dr. Timothy Griffin and Co-directed by Dr. Benjamin Miller. Both directors have over 20 years of experience in studies of metabolism and previous experience in leadership positions. The Core has a proposed structure that facilitates clearly-defined roles of support staff as well as transparent decision-making processes. Through this structure, the Core will foster a mentoring and collaborative culture, promote an idea- and method-sharing environment, and contribute to the establishment of a sustainable resource for metabolic research for COBRE Investigators as well as other researchers in Oklahoma.

俄克拉荷马州细胞代谢研究中心 (CMRO) I 期 COBRE 提案的目标是培养以代谢为重点的新型多学科研究。作为这一总体目标的一部分，我们创建了代谢表型核心促进我们的 COBRE 研究人员过渡到独立的 R01 资助并在俄克拉荷马州开展代谢研究。 COBRE 研究人员提出的项目旨在了解细胞代谢过程驱动或导致心血管疾病的机制，自身免疫性疾病和癌症。由于细胞代谢非常复杂，会产生许多生物学和技术挑战，核心将提供一支由训练有素的主任和博士级科研人员组成的团队，精心维护的最先进仪器以及实验设计方面的指导培训，代谢数据的实施和解释。核心解决了支持我们的三个关键需求 COBRE 研究人员：1) 评估细胞呼吸和线粒体功能，2) 同位素代谢示踪剂分析，3) 基于代谢物的代谢谱和生物信息学分析。为了满足这些需求，我们的具体目标是：1) 提供研究设计、测定优化、数据分析方面的专业知识，以及结果解释，以及评估和开发体内代谢表型的新方法， 2) 为 COBRE 调查人员提供集中设备和适当科学技术的访问权限培训以促进细胞代谢评估新专业知识的发展。支持 COBRE 研究人员，核心将为细胞呼吸和线粒体功能提供仪器（例如，线粒体功能）。 Seahorse、Oroboros 和 EPR）、代谢物示踪分析 (LC-MS/MS) 和代谢分析（GC-MS 和气相色谱-质谱/质谱）。《核心》将由蒂莫西·格里芬博士执导，本杰明·米勒博士联合执导。两个都董事们拥有超过20年的新陈代谢研究经验以及领导经验职位。该核心提出了一个拟议的结构，该结构有助于明确界定支持人员的角色以及透明的决策过程。通过这种结构，核心将促进指导和协作文化，促进思想和方法共享环境，并为建立可持续发展做出贡献为 COBRE 研究人员以及俄克拉荷马州其他研究人员提供代谢研究资源。