METAMORPHIC REGULATION BY THE DROSOPHILA BROAD COMPLEX

果蝇广泛复合体的变态调控

• 批准号: 2187970
• 负责人: JAMES W FRISTROM
• 金额: $ 22.22万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-01-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2187970
• 关键词: DNA binding protein; DNA footprinting; Drosophilidae; RNase protection assay; developmental genetics; ecdysone; gene complementation; gene deletion mutation; gene expression; genetic transcription; hormone regulation /control mechanism; in situ hybridization; metamorphosis; molecular cloning; polymerase chain reaction; protein structure; protein structure function; recombinant DNA; regulatory gene

The Broad-Complex (Br-C) is a Drosophila regulatory gene. Its transcription is controlled by a steroid hormone (20-hydroxyecdysone). The Br-C encodes a set of four DNA-binding Zn finger proteins (Z1, Z2, Z3, Z4) that participate in the hierarchial transcriptional regulation essential for metamorphosis. The Br-C is one of at least such genes that govern metamorphosis. Different Zn finger proteins are produced by the Br-C through differential splicing of Zn finger coding exons to a common "core." Our current results demonstrate that different Br-C Zn finger proteins have different roles in metamorphosis, but they may also be functionally related in a redundant, cooperative and/or competitive manner. Other evidence suggests that the locus may regulate itself both positively and negatively. Additionally, some proteins are expressed in some tissues but not in others, e.g. Z2 and Z4 are transcribed in imaginal disc but not in salivary gland. Finally, the timing of expression of the different transcripts within a given tissue differs. The studies we propose are intended to illuminate the roles this gene and its component parts have in the propagation of a complex hormonally regulated developmental process. Accordingly, we propose to define the functional relationships between Br-C proteins through mutant analyses and induced expression of different Zn finger proteins. A most important objective is to investigate possible autoregulation and cross-regulation at the locus. These studies will be facilitated by the systematic in situ mutagenic dissection of the Br-C to produce mutants that on the one hand, are true null mutations for each Zn finger domain, or, on the other hand, can express only one Zn finger protein. These studies will help to describe the general nature of hormone control of gene expression and development.

Broad-Complex (Br-C) 是果蝇调控基因。 它是 转录由类固醇激素（20-羟基蜕皮激素）控制。 Br-C 编码一组四种 DNA 结合锌指蛋白（Z1、Z2、 Z3, Z4) 参与层级转录调控 对于变态至关重要。 Br-C 至少是这样的基因之一 控制变态。 不同的锌指蛋白由以下物质产生 Br-C通过Zn指编码外显子的差异剪接至a 共同的“核心”。 我们目前的结果表明，不同的 Br-C Zn 手指蛋白在变态中具有不同的作用，但它们也可能 在冗余、合作和/或竞争中具有功能相关性 方式。 其他证据表明该位点可能会自我调节 积极和消极。 此外，一些蛋白质表达于 有些组织，但其他组织则不然，例如Z2 和 Z4 转录为 成虫盘但不在唾液腺中。 最后，时间安排 给定组织内不同转录本的表达是不同的。 我们提出的研究旨在阐明该基因和 它的组成部分具有复杂荷尔蒙传播的作用 规范的发育过程。 因此，我们建议定义 通过突变体分析了解 Br-C 蛋白之间的功能关系 并诱导不同锌指蛋白的表达。 一个最重要的 目标是调查可能的自动监管和交叉监管 在轨迹处。 系统化的研究将促进这些研究 Br-C 的原位诱变解剖产生的突变体一方面 一方面，是每个锌指结构域的真正无效突变，或者，另一方面 hand，只能表达一种锌指蛋白。 这些研究将有助于 描述激素控制基因表达的一般性质和 发展。