Sleep Based Memory Consolidation in Juvenile Zebra Finch

幼年斑胸草雀基于睡眠的记忆巩固

基本信息

批准号：
10319443
负责人：
Danyal H Alam
金额：
$ 3.75万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-01 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10319443
关键词：
Acute Adolescent Adult Affect Animal Model Award Behavior Birds Brain Brain region CRISPR/Cas technology Cell Nucleus Complex Courtship Data Set Development Dissection Engineering Environment Evolution Female Gene Delivery Generations Genes Genetic Engineering Genetic Techniques Goals Hippocampus (Brain)Hour Human Individual Investigation Knowledge Label Lead Learning Ligands Literature Machine Learning Mate Selections Memory Mentors Methods Molecular Motor Nervous system structure Neurons Neurosciences Partner in relationship Pathway interactions Perceptual learning Performance Pharmacology Phase Play Population Postdoctoral Fellow Principal Investigator Process Production RNA Interference Research Research Personnel Research Project Grants Research Training Role Signal Transduction Sleep Sleep disturbances Songbirds Speech Speech Disorders Stereotyping Structure Synapses System Technical Expertise Techniques Testing Therapeutic Toxic effect Training Tropism Uncertainty Viral Viral Vector Virus Work auditory feedback base bird song deep learning fitness follow-up genetic manipulation in vivo infection rate innovation learned behavior male memory consolidation motor behavior neural circuit nonhuman primate novel pathogenic virus post-doctoral training pre-doctoral preference pressure procedural memory theories tool transcriptome sequencing tutoring vector vocal learning vocalization zebra finch

项目摘要

Project Summary Sleep is thought to play a significant contribution in the consolidation of multiple forms of memories during development. Transient disruptions of sleep often lead to delays and losses in learned skilled behaviors. While research has made great progress in understanding the role of sleep in the hippocampal dependent learning of explicit memories, how sleep influences the synaptic circuits involved in learning perceptual and procedural memories is still poorly understood. Songbirds provide an unmatched opportunity to study sleep based consolidation of skilled motor behaviors. Juvenile songbirds learn their courtship song through extensive daily practice, producing tens of thousands of renditions by adulthood. Daily, auditory-feedback guided improvements in song are hypothesized to be consolidated nightly during sleep, enabling renewed vocal exploration and continued refinement of song performances through development. My predoctoral research seeks to identify the synaptic circuits involved in sleep-based consolidation of song and the long-term impact of transient disruptions in these circuits during song development. By combining in vivo pharmacological manipulations with machine learning-based approaches for segregating and analyzing changes in singing behavior, I have identified at least one nuclei governing the sleep-based consolidation of complex motor behaviors. Remaining work to be done during the F99 phase will focus on the specific contributions of individual premotor synaptic circuits using synapse-specific pharmacology in conjunction with pathway specific chemogenetics. Also during the F99 phase, I will follow up on unexpected findings to determine the evolutionary relevance of song diversity in mate choice. The K00 phase of this proposal will provide a specific plan to find an ideal mentor and research environment for enhancing my knowledge and technical skills of neuroinvasion and propagation of viruses leading to generation of novel viral vectors for the effective dissection of neural circuits. Together, the research and training detailed in the two phases of this proposal will allow me to achieve my long-term goal of engineering sophisticated tools to understand the molecular, cellular, and synaptic basis of learned complex motor behaviors as a tenured principal investigator.

项目概要睡眠被认为在巩固多种形式记忆方面发挥着重要作用发展。短暂的睡眠中断通常会导致习得的技能行为的延迟和丧失。尽管研究在理解睡眠在海马依赖性学习中的作用方面取得了很大进展外显记忆，睡眠如何影响参与学习感知和程序的突触回路记忆仍然知之甚少。鸣禽为研究睡眠提供了无与伦比的机会巩固熟练的运动行为。幼年鸣禽通过大量的日常学习来学习求偶歌曲练习，成年后已创作出数以万计的表演。每日听觉反馈引导改进假设歌曲中的声音在每晚睡眠期间得到巩固，从而能够重新进行声音探索和通过开发不断完善歌曲表演。我的博士前研究旨在确定突触回路参与基于睡眠的歌曲巩固以及短暂中断的长期影响在歌曲开发期间在这些电路中。通过将体内药理操作与机器相结合基于学习的方法来分离和分析歌唱行为的变化，我至少已经确定一个控制基于睡眠的复杂运动行为巩固的核心。剩余工作有待完成在 F99 阶段将重点关注各个前运动突触回路的具体贡献，使用突触特异性药理学与途径特异性化学遗传学相结合。同样在F99阶段，我将跟进意想不到的发现，以确定歌曲多样性在择偶中的进化相关性。该提案的K00阶段将提供一个具体的计划来寻找理想的导师和研究环境增强我的神经侵袭和病毒传播的知识和技术技能，从而导致产生用于有效解剖神经回路的新型病毒载体。研究和培训共同详细在这个提案的两个阶段中，我将能够实现设计复杂工具的长期目标理解习得的复杂运动行为的分子、细胞和突触基础首席研究员。