NEOLACTO SERIES OF GLYCOLIPIDS IN DEVELOPING NERVOUS SYSTEM

神经系统发育中的新乳糖系列糖脂

• 批准号: 3735113
• 负责人: FIROZE B JUNGALWALA
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER CTR MTL RETARDATN
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3735113
• 关键词: N acetylglucosaminidase; Schwann cells; animal tissue; antibody; beta galactosidase; cell adhesion; cell cell interaction; cell differentiation; ceramides; cerebellum; cerebral cortex; developmental neurobiology; embryo /fetus cell /tissue; enzyme mechanism; galactosyltransferases; glucuronides; glycolipids; glycosphingolipids; immunocytochemistry; immunoelectron microscopy; injury; laboratory mouse; laboratory rabbit; laboratory rat; monoclonal antibody; mutant; myelination; nervous system regeneration; neural plasticity; peripheral nervous system; radiotracer; sialyltransferases; tissue /cell culture

Neolacto series of glycolipids are expressed in the cortex only during embryonic development but disappear post-natally. These glycolipids however, continue to express themselves in cerebellum and peripheral nervous system (PNS) even in the adulthood. The major goal of this project is to determine the physiological significance of these glycolipids in the processes of cellular differentiation, cell-adhesion, plasticity and regeneration. Four specific aims are described: (1) To understand the regulation of their expression at the enzymic level (2) To evaluate if these glycolipids are involved in regeneration and remyelination processes of PNS, after nerve injury (3) To determine their precise cellular and subcellular localization in situ by light and electron microscopic studies, with the use of monoclonal antibodies and neurological mutants (4) To determine their role in cellular interaction using in vitro model systems. The studies should generate valuable information on the role of these glycolipids in cellular proliferation and migration during development of the embryonic cortex. Their possible role in plastic processes in cerebellum, and regenerative processes in PNS are being considered and evaluated.

Neolacto系列的糖脂系列仅在皮质中表达 胚胎发育，但后产后消失。 这些糖脂 但是，继续在小脑和外围表达自己 神经系统（PNS）即使在成年期也是如此。 主要目标 项目是确定这些的生理意义 在细胞分化，细胞粘附的过程中糖脂 可塑性和再生。 描述了四个具体目标： （1）了解其在酶水平上表达的调节 （2）评估这些糖脂是否参与再生和 神经损伤后PNS的再髓式过程 （3）确定其精确的细胞和亚细胞定位 通过光和电子显微镜研究的原位，使用 单克隆抗体和神经突变体 （4）使用体外模型确定它们在细胞相互作用中的作用 系统。 这些研究应产生有关这些作用的宝贵信息 发育过程中细胞增殖和迁移的糖脂 胚胎皮质。 它们在塑料过程中的可能作用 小脑和PN中的再生过程正在考虑，并且 评估。