Core C: Genomics and Transcriptomics

核心 C：基因组学和转录组学

基本信息

批准号：
10304092
负责人：
Jennifer S Yokoyama
金额：
$ 30.65万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-27 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10304092
关键词：
Alzheimer&apos s Disease Amyotrophic Lateral Sclerosis Antisense Oligonucleotides Bioinformatics Biological Biological Process Biology C9ORF72 Candidate Disease Gene Cell Nucleus Cell physiology Clinical Collaborations Communities Data Data Set Databases Development Diagnosis Disease Elderly Family Foundations Frontotemporal Dementia Frontotemporal Lobar Degenerations Gene Mutation Genes Genetic Genetic Risk Genetic Variation Genomics Goals Homeostasis Human Human Genetics Inherited Knowledge Link MAPT gene Metabolism Molecular Chaperones Mutation Nerve Degeneration Neurodegenerative Disorders Parkinson Disease Pathogenicity Pathologic Pathway interactions Patients Peptide Hydrolases Pick Disease of the Brain Progressive Supranuclear Palsy Proteins Proteolysis Recording of previous events Research Risk Role Sampling Science Site Tauopathies Translations Update Variant corticobasal degeneration data sharing disorder risk frontotemporal lobar dementia-amyotrophic lateral sclerosis genetic risk factor genome sequencing human disease human genomics insight multidisciplinary neuropathology new therapeutic target novel polygenic risk score prevent protein TDP-43 proteostasis success synergism targeted treatment tau Proteins tau function therapeutic development tool transcriptomics whole genome

项目摘要

PROJECT SUMMARY: CORE C: GENOMICS AND TRANSCRIPTOMICS The primary objective of Core C is to support the genomics and transcriptomics needs of the U54 FTD Center without Walls. Utilizing pre-existing whole genome sequencing data from 721 patients with neu- rodegenerative disease and healthy older adult controls, this Core will provide foundational information regarding variation in tau metabolism pathway genes that will be directly interrogated by the Center through its Projects. Additional gene candidates nominated by the Center will be screened by Core C for relevance to human neurodegenerative disease. In parallel, this Core will generate a `tau polygenic risk score' (TPRS) that will be used to stratify samples at high versus low genetic risk for tau-mediated neu- rodegeneration. These samples will be studied by Project 2 to elucidate the cellular processes by which tau is dysregulated in patients with varying degrees of genetic risk for tau-mediated neurodegeneration. Core C will also support the bioinformatics and statistical needs of the Projects throughout the study period, including analysis of bulk and single-nucleus transcriptomics data generated by Project 2. All of this data will be integrated into an informational database, the `Tau Metabolism and Variant database' (TMVdb), in collaboration with administrative Core A. The TMVdb will be regularly updated and both it and the data used to generate it will be made available to the research community through an analytical workbench interface. All of the Core's proposed activities will contribute to the Center's primary goal of understanding how human genetic variation contributes to tau metabolism and homeostasis and will be critical to its success.

项目摘要：核心 C：基因组学和转录组学 Core C 的主要目标是支持 U54 FTD 的基因组学和转录组学需求没有围墙的中心。利用来自 721 名神经性神经病患者的现有全基因组测序数据退化性疾病和健康的老年人控制，该核心将提供基础信息关于将由中心直接询问的 tau 代谢途径基因的变异通过其项目。中心提名的其他候选基因将由 Core C 筛选与人类神经退行性疾病的相关性。同时，该核心将产生“tau 多基因风险” 评分”（TPRS）将用于对 tau 介导的神经细胞遗传风险高与低的样本进行分层退化。项目 2 将研究这些样本，以阐明细胞过程在具有不同程度 tau 介导的神经变性遗传风险的患者中，tau 蛋白失调。核心 C 还将在整个研究过程中支持项目的生物信息学和统计需求期间，包括对项目 2 生成的批量和单核转录组数据的分析。所有该数据将被整合到信息数据库“Tau 代谢和变异数据库”中 (TMVdb)，与管理核心 A 合作。TMVdb 将定期更新，并且用于生成数据的数据将通过分析提供给研究界工作台界面。核心提议的所有活动都将有助于实现该中心的主要目标：了解人类遗传变异如何促进 tau 代谢和稳态对其成功至关重要。