GERM CELL DIFFERENTIATION IN VITRO

生殖细胞体外分化

• 批准号: 2196381
• 负责人: LAURA L RICHARDSON
• 金额: $ 2.37万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-09 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2196381
• 关键词: GERM; CELL; DIFFERENTIATION; VITRO; GERM; CELL; DIFFERENTIATION; VITRO

During spermatogenesis, germ cells make several important developmental decisions. One of the most important is the choice to cease mitotic proliferation and enter meiosis. Much has been learned about the switch from mitosis to meiosis in lower organisms, but the study of the molecules in the meiotic pathway and the factors which regulate them in mammalian germ cells has been more difficult. The recent development of mouse testicular cell lines, including two germ cell lines which undergo meiosis in vitro, now make molecular studies of mammalian germ cell differentiation more feasible. The experiments outlined in this proposal will test the hypothesis that Sertoli cells influence the decision of germ cells to enter meiosis. Cocultures of the somatic and germ cell lines will be used to determine the impact of Sertoli cells on the initiation of meiosis. Differential display of expressed mRNAs and recombinant antibodies will be used to identify molecules involved in the transition from mitosis to meiosis. Specific aim one will examine the effects of Sertoli cells on the initiation of meiosis by the germ cells and will identify molecules involved in the communication between the Sertoli and germ cells which potentially regulate the entry of the germ cells into meiosis. The second specific aim will identify molecules that are expressed in germ cells as they leave the mitotic cell cycle and enter the meiotic pathway. Finally, in specific aim three, recombinant antibodies will be produced to germ cell surface proteins to obtain reagents to early differentiation markers and identify additional molecules which may be involved in the regulation of germ cell differentiation. These experiments will provide insight into the molecular mechanisms responsible for mammalian germ cell differentiation.

在精子发生过程中，生殖细胞产生了几种重要的发育 决定。 最重要的之一是选择停止有丝分裂 扩散并进入减数分裂。 关于开关已经有很多了解 从有丝分裂到较低生物体的减数分裂，但研究 减数分裂途径中的分子以及调节它们的因素 哺乳动物的生殖细胞更加困难。 最近的发展 小鼠睾丸细胞系，包括两种经历的生殖细胞系 体外减数分裂，现在进行哺乳动物生殖细胞的分子研究 区分更可行。 该提案中概述的实验 将检验以下假设，即Sertoli细胞会影响 生殖细胞进入减数分裂。 体细胞和生殖细胞的共培养 线将用于确定Sertoli细胞对 减数分裂的启动。 表示mRNA的差分显示和 重组抗体将用于鉴定参与 从有丝分裂到减数分裂的过渡。 特定目标将检查 Sertoli细胞对生殖细胞减数分裂的影响 并将确定与 Sertoli和生殖细胞可能调节生殖的进入 细胞进入减数分裂。 第二个特定目标将确定分子 在生殖细胞离开有丝分裂细胞周期和 进入减数分裂途径。 最后，在特定目标中，重组 将对生殖细胞表面蛋白产生抗体以获得 早期分化标记的试剂并确定其他 可能与生殖细胞调节有关的分子 分化。 这些实验将为您提供有关 负责哺乳动物生殖细胞分化的分子机制。