STEROID AND SENSORY INFLUENCES ON NORADRENERGIC RELEASE

类固醇和感官对去甲肾上腺素能释放的影响

基本信息

批准号：
2242413
负责人：
JEANNIE M FIBER
金额：
$ 2.26万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-03-05 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/2242413
关键词：
STEROID SENSORY INFLUENCES NORADRENERGIC RELEASE

项目摘要

The experiments in this proposal focus on the effects of gonadal steroids and sensory stimuli on noradrenergic (NE) transmission in brain regions that mediate reproductive behavior in female rats. Steroid-regulated NE input into both the ventromedial hypothalamus (VMH) and medial preoptic area (MPOA) may help coordinate reproductive function and may regulate other neurotransmitters known to influence reproductive behavior within these brain regions. The objectives of these experiments are 1) to determine which aspects of sensory input associated with reproductive behavior (chemosensory or somatosensory) contribute to hypothalamic NE release; 2) to determine the steroid dependence and stimulus dependence of NE transmission in the MPOA. in vivo brain microdialysis will be used in awake, behaving animals to accomplish goals l and 2; and 3) to determine the role of NE and gonadal steroids in regulating GABA release in the POA and hypothalamus, and to determine which NE receptor subtype mediates any interaction between NE and GABA. This will be achieved using in vitro release studies on brain tissue slices using steroid treatments and pharmacological manipulations. This research project is aimed at determining NE and GABA interactions, sensory influences on NE release, and steroid influences on NE release. These experiments have implications for understanding the mechanisms underlying human reproduction and affective disorders such as depression, since both NE and GABA and interactions between steroids and these neurotransmitters are involved in these phenomena.

该提案中的实验重点关注性腺类固醇的影响和感觉刺激对大脑区域去甲肾上腺素能（NE）传输的影响介导雌性大鼠的生殖行为。类固醇调节的NE 输入到下丘脑腹内侧 (VMH) 和内侧视前区区域（MPOA）可能有助于协调生殖功能并可能调节其他已知影响生殖行为的神经递质这些大脑区域。这些实验的目的是 1) 确定感觉输入的哪些方面与生殖相关行为（化学感觉或体感）导致下丘脑 NE 发布; 2）确定类固醇依赖性和刺激依赖性 MPOA 中的 NE 传输。将使用体内脑微透析清醒时，动物表现出色以实现目标 l 和 2； 3) 至确定 NE 和性腺类固醇在调节 GABA 释放中的作用在 POA 和下丘脑中，并确定哪种 NE 受体亚型介导 NE 和 GABA 之间的任何相互作用。这将通过使用来实现使用类固醇治疗脑组织切片的体外释放研究和药理操作。该研究项目旨在确定 NE 和 GABA 相互作用、感官对 NE 释放的影响，和类固醇对 NE 释放的影响。这些实验具有重要意义为了了解人类生殖的机制和情感障碍，例如抑郁症，因为 NE 和 GABA 都涉及类固醇和这些神经递质之间的相互作用在这些现象中。