Investigating nanoscale neuronal damages in early glaucoma towards clinical optical detection

研究早期青光眼的纳米级神经元损伤以进行临床光学检测

基本信息

批准号：
10228558
负责人：
Xiaorong Liu
金额：
$ 50.75万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-06-01 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10228558
关键词：
Acute Anatomy Animal Model Animals Atrophic Axon Behavior Biological Markers Blindness Caring Cell Death Cell Density Cell Survival Cessation of life Chronic Clinical Clinical Management Contrast Sensitivity Crush Injury Defect Dendrites Detection Diagnosis Disease Management Early Diagnosis Electroretinography Exhibits Experimental Models Foundations Glaucoma Goals Healthcare Systems Human Image Imaging technology Individual Investigation Label Left Length Light Measures Methodology Modeling Molecular Monitor Mus Nerve Crush Neurons Optic Nerve Optic Nerve Injuries Optical Coherence Tomography Optics Patient imaging Patients Phenotype Physiologic Intraocular Pressure Physiological Property Reporter Research Personnel Resolution Retina Retinal Ganglion Cells Risk Structure Symptoms System Techniques Technology Thick Thinness Time Transgenic Mice Translating Visible Radiation Vision Vision Disorders Visual Work axon injury cell injury clinical Diagnosis clinical application clinical imaging contrast imaging design early screening economic impact fundus imaging human model imaging modality improved in vivo medical attention mouse model nanoscale neuronal cell body operation retinal nerve fiber layer technology development technology validation tool

项目摘要

Project Summary Our long-term goal is to develop new functional extensions of optical coherence tomography (vis-OCT) for clinical diagnosis and management of glaucoma. Glaucoma is characterized by a progressive loss of retinal ganglion cells (RGCs) and optic nerve defects and atrophy, afflicting more than 60 million people worldwide. It is often develops slowly with symptoms and damage that are not noticed at early stage. Caring for those with glaucomatous vision disability poses a huge burden on the US health care system. Early detection and screening techniques at asymptomatic stage is thus of critical importance for better management of the disease and will have paramount clinical and economic impacts. In this proposal, the power of the ultrahigh axial resolution (~1 µm) will be harnessed and the increased optical contrast sensitivity offered by visible-light OCT (vis-OCT) to investigate the mechanism of optically detectable alterations underlying early retinal ganglion cell (RGC) damage in mouse models of experimental glaucoma. The proposed study will serve as the foundation for objective and sensitive early clinical diagnosis of glaucoma using OCT. First, the investigators will establish vis-OCT methodology to detect RGC and axon damage using an acute mouse model of nerve crush injury. Then, the investigators will determine physiological origin of vis-OCT detected RGC ultrastructural alterations in a chronic model of experimental glaucoma. Finally, the investigators will determine that vis-OCT detected RGC ultrastructural alterations proceeds the structural and functional metrics used for clinical glaucoma diagnoses in a chronic model of experimental glaucoma.

项目概要我们的长期目标是开发光学相干断层扫描（vis-OCT）的新功能扩展青光眼的临床诊断和治疗的特点是视网膜进行性丧失。神经节细胞 (RGC) 和视神经缺陷和萎缩，困扰着全世界超过 6000 万人。照顾那些有青光眼视力障碍给美国医疗保健系统带来了巨大的负担。因此，无症状阶段的筛查技术对于更好地管理疾病至关重要在该提案中，超高轴的力量将产生重大的临床和经济影响。将利用可见光 OCT 提供的分辨率（~1 µm）和增强的光学对比灵敏度（vis-OCT）研究早期视网膜神经节细胞光学可检测改变的机制 (RGC) 实验性青光眼小鼠模型中的损伤本研究将作为以下研究的基础。首先，研究人员将建立使用 OCT 进行青光眼客观且敏感的早期临床诊断。使用急性小鼠神经挤压损伤模型检测 RGC 和轴突损伤的 vis-OCT 方法。然后，研究人员将确定 vis-OCT 检测到的 RGC 超微结构改变的生理起源。最后，研究人员将确定 vis-OCT 检测到 RGC。超微结构的改变促进了用于临床青光眼诊断的结构和功能指标实验性青光眼的慢性模型。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Multicolor super-resolution imaging using spectroscopic single-molecule localization microscopy with optimal spectral dispersion.

使用具有最佳光谱色散的光谱单分子定位显微镜进行多色超分辨率成像。

DOI：
10.1364/ao.58.002248
发表时间：
2019-03-15
期刊：
Applied optics
影响因子：
1.9
作者：
Yang Zhang;Ki;B. Dong;Janel L. Davis;Guangbin Shao;Cheng Sun;Hao F. Zhang
通讯作者：
Hao F. Zhang

Alignment of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina.

可见光光学相干断层扫描纤维图与同一小鼠视网膜的共焦图像的对齐。

DOI：
发表时间：
2023-06-30
期刊：
影响因子：
0
作者：
Chang, Shichu;Xu, Wenjin;Fan, Weijia;McDaniel, John A;Grannonico, Marta;Miller, David A;Liu, Mingna;Zhang, Hao F;Liu, Xiaorong
通讯作者：
Liu, Xiaorong

Characterization of retinal ganglion cell damage at single axon bundle level in mice by visible-light optical coherence tomography fibergraphy.

通过可见光光学相干断层扫描纤维成像表征小鼠单轴突束水平的视网膜神经节细胞损伤。

DOI：
发表时间：
2023-01
期刊：
影响因子：
6.1
作者：
Liu, Xiaorong;Zhang, Hao F
通讯作者：
Zhang, Hao F

Machine-learning based spectral classification for spectroscopic single-molecule localization microscopy.

基于机器学习的光谱分类，用于光谱单分子定位显微镜。

DOI：
发表时间：
2019-12-01
期刊：
影响因子：
3.6
作者：
Zhang, Zheyuan;Zhang, Yang;Ying, Leslie;Sun, Cheng;Zhang, Hao F
通讯作者：
Zhang, Hao F

Gene dosage manipulation alleviates manifestations of hereditary PAX6 haploinsufficiency in mice.

基因剂量控制减轻了小鼠遗传性 PAX6 单倍体不足的表现。

DOI：
发表时间：
2020
期刊：
影响因子：
17.1
作者：
Rabiee, Behnam;Anwar, Khandaker N;Shen, Xiang;Putra, Ilham;Liu, Mingna;Jung, Rebecca;Afsharkhamseh, Neda;Rosenblatt, Mark I;Fishman, Gerald A;Liu, Xiaorong;Ghassemi, Mahmood;Djalilian, Ali R
通讯作者：
Djalilian, Ali R