Cell & Molecular Imaging Core

细胞

基本信息

批准号：
10226348
负责人：
Anna K Majewska
金额：
$ 18.5万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10226348
关键词：
Address Algorithms Animal Model Animals Biological Biological Process Biometry Brain Clinical Sciences Collaborations Communities Complex Analysis Computer software Computers Consultations Custom Data Data Analyses Data Collection Data Set Ensure Equipment Experimental Designs Facility Designs Faculty Human Image Image Analysis Imaging Device In Vitro Individual Infrastructure Institutes Intellectual and Developmental Disabilities Research Centers Label Laboratories Laboratory Research Methodology Methods Microscopy Modification Performance Phase Play Process Protocols documentation Reproducibility Research Research Personnel Research Project Grants Resolution Resources Role Sampling Scientist Signal Pathway Standardization System Technical Expertise Techniques Technology Time Tissues Training Translational Research Universities Viral Viral Vector base cellular imaging data acquisition data management data sharing design experience experimental study image processing imaging modality improved in vivo in vivo imaging instrumentation interest member molecular imaging programs spectrograph success theories tool

项目摘要

Project Summary The URMC IDDRC Cell and Molecular Imaging Core (CMI Core) provides IDDRC members with access to advanced imaging, analysis, and viral vector-based transduction methods, as well as corresponding faculty expertise, to support in vitro and in vivo study of phenomena at the subcellular, cellular, tissue, and whole animal scale. The capabilities provided are central to research conducted by IDDRC investigators who have been using the available techniques to study subcellular, cellular, and systems level processes in the developing brain of animal models and humans. The integration of these cutting edge methods with expert faculty guidance ensures and streamlines access to techniques and equipment that are fundamental to IDD research. State-of-the-art imaging equipment and software provides a spectrum of image acquisition and analysis approaches (confocal, multiphoton and super-resolution imaging) and our viral vector facility designs and produces custom viral approaches for both labeling and manipulating signaling pathways for in vitro and in vivo imaging and analysis. The CMI Core is supported by dedicated administrative and technical faculty and staff to provide customized, critical technical expertise in the design, implementation, and analysis of studies involving microscopy that span the interests of the IDDRC faculty. The CMI Core leverages existing expert faculty and staff and long-standing infrastructure (confocal, multiphoton, analysis), with a proven track record of usage by IDDRC investigators, with newer capabilities (super-resolution, viral vectors), which have been implemented based upon recognized needs. The result is a CMI Core that allows IDDRC investigators to design and implement meaningful experiments using cutting edge technologies and efficient analysis paradigms to address difficult and diverse biological problems.

项目概要 URMC IDDRC 细胞和分子成像核心（CMI 核心）为 IDDRC 成员提供获得先进的成像、分析和基于病毒载体的转导方法，以及相应的教师专业知识，以支持对现象的体外和体内研究亚细胞、细胞、组织和整个动物规模。所提供的功能是核心 IDDRC 调查人员进行的研究，他们一直在使用现有技术研究动物模型大脑发育中的亚细胞、细胞和系统水平过程和人类。这些前沿方法与专家教师指导的结合确保并简化 IDD 研究基础技术和设备的获取。最先进的成像设备和软件提供了一系列图像采集和分析方法（共焦、多光子和超分辨率成像）和我们的病毒载体设施设计并生产用于标记和操作的定制病毒方法用于体外和体内成像和分析的信号通路。 CMI 核心由以下支持专门的行政和技术教职员工提供定制的关键技术涉及显微镜研究的设计、实施和分析方面的专业知识 IDDRC 教师的利益。 CMI 核心利用现有的专家教职员工和长期存在的基础设施（共焦、多光子、分析），具有良好的使用记录由 IDDRC 研究人员开发，具有更新的功能（超分辨率、病毒载体），这些功能已根据公认的需求实施。结果是 CMI 核心允许 IDDRC 研究人员使用尖端技术设计和实施有意义的实验和有效的分析范式来解决困难和多样化的生物学问题。