Cryo-Electron Microscopy and Tomography Core

冷冻电子显微镜和断层扫描核心

• 批准号: 10219098
• 负责人: Peijun Zhang
• 金额: $ 30.2万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-27 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10219098
• 关键词: Buffers; Complement; Complex; Core Protein; Cryoelectron Microscopy; Detection; Devices; HIV; HIV-1; Heterogeneity; Methodology; Methods; Microscopic; Molecular Conformation; Morphology; Negative Staining; Particle Size; Play; Proteins; Resolution; Role; Sampling; Structural Protein; Structure; X-Ray Crystallography; base; electron tomography; image reconstruction; improved; instrumentation; particle; protein complex; protein structure

Cryo-EM Core Abstract The Cryo-EM Core will be an important component of the PCHPI structure determination pipeline, playing a major role in Project 1 and significantly contributing to Projects 2, 3 and 4, and tackling new HIV-host protein complexes as they become available. The Core will screen complexes produced in the Protein Core or by collaborators for their suitability for Cryo-EM or Cryo-ET structure determination and will determine the structures of HIV-host protein complexes at atomic or near-atomic resolution, using single particle methodologies. In addition, the Cryo-EM Core will develop methods to analyze structural heterogeneity within samples, an issue that complicates current EM-based analytical approaches.

冷冻电镜核心摘要 Cryo-EM Core 将成为 PCHPI 结构测定流程的重要组成部分，在 在项目 1 中发挥主要作用，对项目 2、3 和 4 做出重大贡献，并解决新的 HIV 宿主蛋白问题 复合物可用时。核心将筛选蛋白质核心中产生的复合物或通过 合作者是否适合 Cryo-EM 或 Cryo-ET 结构测定，并将确定 使用单粒子以原子或近原子分辨率研究 HIV 宿主蛋白复合物的结构 方法论。此外，Cryo-EM Core 将开发分析内部结构异质性的方法。 样品，这个问题使当前基于电磁的分析方法变得复杂。