GROWTH FACTORS--ACTIONS ON TYPE II CELLS IN BPD

生长因子--对 BPD 中 II 型细胞的作用

• 批准号: 2230156
• 负责人: RICHARD J KING
• 金额: $ 15.35万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2230156
• 关键词: baboons; bronchopulmonary dysplasia; cell type; disease /disorder model; fibroblasts; growth factor; growth factor receptors; hepatocyte growth factor; messenger RNA; phenotype; premature infant animal; surfactant; tissue /cell culture; transforming growth factors

DESCRIPTION (adapted from the applicants' abstract): This proposal describes experiments to be conducted on the role of growth factors in the pathogenesis of bronchopulmonary dysplasia (BPD) in the prematurely- delivered neonatal baboon. In previous work we have described a primate model of BPD and used it to study some of the physiological and morphological changes which occur with the development of this disease. Recently, we have documented decreases in the steady state mRNAs of all surfactant proteins in this baboon homolog, correlated these changes with perturbations in morphology and physiology, and have observed a concomitant hyperplasia of type 11 cells. In addition, we have found that in conjunction with the development of neonatal lung injury there is an elevation in the amounts of TGF-A and HGF produced by lung cells. The studies in this proposal are predicated upon these findings, together with preliminary data showing that TGF-A can affect the biochemical activities of type 11 cells, and that HGF can stimulate their proliferation. These experiments will extend these observations specifically toward fetal development and its possible perturbation by premature delivery and the development of BPD. The proposed experiments are designed to accomplish the following Specific Aims: [1] To study the developmental profile of these growth factors and their receptors. [2] To determine if this pattern is altered in BPD. [3] To study the responsiveness of fetal and post-natal type 11 cells to these growth factors.

描述（改编自申请人的摘要）：此提案 描述了有关生长因子在中的作用的实验 过早的支气管肺发育异常（BPD）的发病 传送新生儿狒狒。在以前的工作中，我们描述了灵长类动物 BPD模型，并用它来研究一些生理和 这种疾病的发展发生的形态变化。 最近，我们记录了所有人的稳态mRNA的下降 在这种狒狒同源物中，表面活性剂蛋白将这些变化与 形态学和生理学的扰动，并观察到 11型细胞的伴随增生。此外，我们发现了 与那里的新生儿肺损伤的发展结合 是肺细胞产生的TGF-A和HGF的量的升高。 该提案中的研究基于这些发现，共同 有了初步数据，表明TGF-A会影响生化 11型细胞的活动，HGF可以刺激其 增殖。这些实验将扩展这些观察结果 特别针对胎儿发育及其可能的扰动 过早交付和BPD的发展。提出的实验 旨在实现以下特定目标：[1]研究 这些生长因子及其受体的发展概况。 [2] 确定在BPD中是否改变了此模式。 [3]研究 胎儿和产后11型细胞对这些生长的反应性 因素。