Smart skin grafts for quantitative assessment and treatment of diabetic wounds

用于定量评估和治疗糖尿病伤口的智能植皮

• 批准号: 10218396
• 负责人: Huanyu Cheng
• 金额: $ 61.96万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-18 至 2024-09-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10218396
• 关键词: Adhesions; Affect; Amputation; Animals; Anti-Bacterial Agents; Antifungal Agents; Bacterial Infections; Caring; Chronic; Citrates; Clinical; Clinical Trials; Debridement; Devices; Diabetes Mellitus; Diabetic Foot; Diabetic Foot Ulcer; Electric Stimulation; Electrodes; Electronics; Environment; Evaluation; Excision; Gold; Grant; Health Care Costs; Hydrogels; Infection; Infection Control; Inflammation; Injectable; Intervention; Limb structure; Lower Extremity; Measures; Medical; Methods; Microbiology; Monitor; Patients; Performance; Plantar Ulcers; Process; Provider; Public Health; Quality of life; Research Personnel; Research Proposals; Skin; Skin graft; Temperature; Therapeutic; Tissue Engineering; Tissues; Trace Elements; Ulcer; United States; Visualization; Wound models; Yang; Zinc; base; cell motility; chronic wound; clinically relevant; design; diabetic ulcer; electric field; experience; fungus; healing; improved; in vivo; interactive feedback; keratinocyte; migration; next generation; point of care; pressure; prevent; sensor; wound; wound care; wound closure; wound dressing; wound environment; wound healing; wound treatment

Project Summary Chronic, difficult-to-heal wounds affect the lives of more than 25 million people with diabetes in the United States, leading to increased healthcare costs and decreased quality of life. Patients with late stage diabetic foot ulcers often progress to amputation for a gangrenous lower extremity limb. Therefore, early stage medical intervention is critical for diabetic foot care. Because of pressure relief, the total-contact cast is the gold standard and most widely used method for healing the most common type of plantar ulcerations (grade 1 ulcers). However, the cast needs to be changed periodically for wound inspection and evaluation, which is burdensome to both patients and providers. Therefore, it is desirable to continuously monitor wounds without direct visualization. Furthermore, because the endogenous electric field is askew or absent in chronic diabetic wounds, cell migration is impeded, often rendering standard wound care inadequate. By mimicking the endogenous electric field, electrical stimulation has been shown in several clinical trials to enhance healing and closure. While many smart wound dressings have been developed to monitor the wound environment, they need regular changes, impeding wound healing. Although skin grafts eliminate the need for removal, they lack sensing capabilities and cannot mitigate infections. Here, the investigators propose to develop and validate paradigm-shifting fully resorbable smart tissue-engineering skin grafts for diabetic wound care. The fully resorbable smart tissue-engineering skin grafts could quantitatively assess the wound environment and concurrently deliver electrical stimulation for enhanced wound healing. The PI Dr. Cheng recently developed skin-conformal electronics to continuously monitor the temperature and moisture levels of the wound, along with the fabrication of biodegradable sensors and electrodes with an essential trace element of zinc on a biodegradable substrate. The anti-bacterial/anti-fungal hydrogels recently developed in co-I Dr. Yang’s lab have been shown to have high wet adhesion for sutureless wound closure with nearly no chronic inflammation. As a clinical microbiologist, co-I Dr. Craft with expertise in microbiology will help guide the clinical relevance. In addition to co-I Dr. Ravnic’s extensive experiences on animal and patient studies with infected diabetic wounds, MD Ulbrecht will also contribute his expertise in diabetic foot care. The investigators will first develop and validate Zn-based temperature/moisture sensors on the anti-bacterial/anti- fungal bioadhesive substrate. After integrating the Zn-based electrode on the bioadhesive, the investigators will investigate the therapeutic function of the smart skin graft via electrical stimulation because of infection reduction and electric field-facilitated migration and proliferation for diabetic wound care. The smart skin graft will substantially improve wound care by quantitative assessment and enhanced treatment of chronic wounds, which can result in next-generation wound care devices to replace the existing wound dressings or skin grafts.

项目概要 慢性、难以愈合的伤口影响着美国超过 2500 万糖尿病患者的生活， 导致晚期糖尿病足溃疡患者的医疗费用增加和生活质量下降。 下肢坏疽往往会进展到截肢，因此需要早期进行医疗干预。 对于糖尿病足部护理至关重要，因为可以缓解压力，全接触石膏是黄金标准，也是最重要的。 广泛用于治疗最常见类型的足底溃疡（1 级溃疡）的方法。 需要定期更换石膏以进行伤口检查和评估，这对患者来说都是负担 因此，需要在不直接观察的情况下连续监测伤口。 此外，由于慢性糖尿病伤口中内源电场倾斜或不存在，细胞迁移 受到阻碍，通常会导致标准伤口护理不充分。 多项临床试验表明，电刺激可以增强愈合和闭合效果。 伤口敷料已被开发用于监测伤口环境，它们需要定期更换，妨碍 尽管皮肤移植消除了去除的需要，但它们缺乏传感能力并且不能愈合。 在这里，研究人员建议开发并验证范式转变的完全可吸收的。 用于糖尿病伤口护理的智能组织工程皮肤移植物完全可吸收的智能组织工程皮肤。 移植物可以定量评估伤口环境，同时提供电刺激 增强伤口愈合。 PI Cheng 博士最近开发了皮肤适形电子器件，可以连续监测温度和 伤口的湿度水平，以及可生物降解的传感器和电极的制造 最近在可生物降解的基质上添加了锌的必需微量元素。 杨博士实验室开发的产品已被证明具有高湿粘合力，可用于无缝线伤口闭合 几乎没有慢性炎症，具有微生物学专业知识的联合教授 Craft 博士将提供帮助。 除了 Ravnic 博士在动物和患者研究方面的丰富经验之外，还指导临床相关性。 对于感染糖尿病伤口，Ulbrecht 医学博士还将贡献他在糖尿病足护理方面的专业知识。 研究人员将首先开发和验证基于锌的温度/湿度传感器的抗菌/抗 将锌基电极集成到生物粘合剂上后，研究人员将进行真菌生物粘附基质的研究。 研究智能皮肤移植通过电刺激减少感染的治疗功能 电场促进的迁移和增殖用于糖尿病伤口护理。 通过定量评估和加强对慢性伤口的治疗，显着改善伤口护理， 可以产生下一代伤口护理设备来取代现有的伤口敷料或皮肤移植物。

项目成果

Iontronic pressure sensor with high sensitivity over ultra-broad linear range enabled by laser-induced gradient micro-pyramids.

离子电子压力传感器通过激光诱导梯度微金字塔实现超宽线性范围内的高灵敏度。

• 发表时间: 2023-06-01
• 影响因子: 16.6
• 作者: Yang, Ruoxi;Dutta, Ankan;Li, Bowen;Tiwari, Naveen;Zhang, Wanqing;Niu, Zhenyuan;Gao, Yuyan;Erdely, Daniel;Xin, Xin;Li, Tiejun;Cheng, Huanyu
• 通讯作者: Cheng, Huanyu

A wireless, solar-powered, optoelectronic system for spatial restriction-free long-term optogenetic neuromodulations.

一种无线、太阳能供电的光电系统，用于无空间限制的长期光遗传学神经调节。

• 发表时间: 2023-09-29
• 影响因子: 13.6
• 作者: Park, Jaejin;Kim, Kyubeen;Kim, Yujin;Kim, Tae Soo;Min, In Sik;Li, Bowen;Cho, Young Uk;Lee, Chanwoo;Lee, Ju Young;Gao, Yuyan;Kang, Kyowon;Kim, Do Hyeon;Choi, Won Jun;Shin, Hyun;Kang, Ho Kwan;Song, Young Min;Cheng, Huanyu;Cho, Il
• 通讯作者: Cho, Il

Skin-Interfaced Bifluidic Paper-Based Device for Quantitative Sweat Analysis.

用于定量汗液分析的皮肤界面双流体纸基装置。

• 发表时间: 2024-03
• 作者: Deng, Muhan;Li, Xiaofeng;Song, Kui;Yang, Hanlin;Wei, Wenkui;Duan, Xiaojun;Ouyang, Xiaoping;Cheng, Huanyu;Wang, Xiufeng
• 通讯作者: Wang, Xiufeng

Superhydrophobic, stretchable kirigami pencil-on-paper multifunctional device platform.

超疏水、可拉伸剪纸纸笔多功能装置平台。

• DOI: 10.1016/j.cej.2023.142774
• 发表时间: 2023-04-01
• 期刊: Chemical engineering journal
• 影响因子: 15.1
• 作者: Ye Xue;Zihan Wang;Ankan Dutta;Xue Chen;P. Gao;Runze Li;Jiayi Yan;Guangyu Niu;Ya Wang
• 通讯作者: Ya Wang

Highly Sensitive Wearable Sensor Based on (001)-Orientated TiO2 for Real-Time Electrochemical Detection of Dopamine, Tyrosine, and Paracetamol.

基于 (001) 取向 TiO2 的高灵敏度可穿戴传感器，用于实时电化学检测多巴胺、酪氨酸和扑热息痛。

• DOI: 10.1002/smll.202312238
• 发表时间: 2024-02-06
• 期刊: Small
• 影响因子: 13.3
• 作者: Tianyao Zhang;Jia Zhu;Maowen Xie;Ke Meng;Guang Yao;T. Pan;Min Gao;H. Cheng;Yuan Lin
• 通讯作者: Yuan Lin