Metabolism Core

新陈代谢核心

基本信息

批准号：
10271866
负责人：
ANDREW N. LANE
金额：
$ 20.32万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-03-01 至 2026-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10271866
关键词：
Address Algorithms Behavior Biochemical Biochemistry Bioinformatics Shared Resource Biological Biology Biomass Biometry Cancer Center Carbon Cells Centers of Research Excellence Chromatography Classification Collaborations Consult Culture Media Data Data Analyses Databases Development Dinucleoside Phosphates Disease Electrophoresis Experimental Designs Fourier Transform Genomics Goals Human Resources Inductively Coupled Plasma Mass Spectrometry Informatics Infusion procedures Isotope Labeling Isotopes Kentucky Liquid Chromatography Literature Logistic Regressions Malignant Neoplasms Mass Spectrum Analysis Metabolic Metabolic Pathway Metabolism Methods Mus Nuclear Magnetic Resonance Operative Surgical Procedures Organism Output Oxidation-Reduction Pathway Analysis Phase Preparation Principal Investigator Procedures Process Protein Analysis Protein Array Proteins Research Personnel Resolution Retrieval Sampling Schedule Services Source Standardization Statistical Data Interpretation System Techniques Technology Time Tissue Harvesting Tissues Tracer Universities Update Validation advanced analytics analytical method base cell growth data acquisition data reduction design experimental study high throughput analysis medical specialties metabolic abnormality assessment metabolome metabolomics stable isotope tumor metabolism ultra high resolution web site

项目摘要

PROJECT SUMMARY “Metabolomics” largely consists of metabolic profiling, which is the process of identification and quantification of a large number of metabolites of many different biochemical classes, from diverse biological samples, including cells, culture media, tissue and biofluids. However, to understand the metabolic underpinning of diseases such as cancer, biochemical mechanism(s) must be elucidated, in which case it is necessary to introduce appropriate stable isotope tracers. Although this greatly increases the number of analytes to be quantified, it also enables the retrieval of much more biologically relevant information. This Stable Isotope Resolved Metabolomics (SIRM) approach is a unique specialty of the Metabolism Core, led by PIs with over 40 years of combined expertise in SIRM. The goals of the Metabolism Core are to provide services for a wide coverage of the metabolome with isotopomer and isotopologue analysis, and high throughput protein analysis. To achieve this, we make use of four major analytical platforms, namely (1) high resolution nuclear magnetic resonance (NMR), (2) ultra-high resolution Fourier-transform mass spectrometry (UHR-FTMS), with or without chromatography, (3) ultra-trace elemental analyses by inductively-coupled plasma MS (ICPMS), and (4) Reverse Phase Protein Arrays (RPPA). The Metabolism Core personnel will advise on analytical methods and SIRM-based experimental design with development of additional techniques to address specific issues of identification and quantification and biological interpretation relevant to the COBRE Projects. Because experimental design and sample preparation are integral to the data acquisition and interpretation, the Metabolism Core provides expertise in these and informatics relevant to the SIRM approach. These goals will be achieved according to the following Specific Aims. Specific Aim 1. To provide access and expertise across a wide range of NMR, MS and RPPA technologies to investigators. Specific Aim 2. To provide experimental design, sample processing, and support to the investigators for data reduction, metabolic pathway, and biochemical mechanism analyses. Specific Aim 3. To develop and implement new methods to enhance metabolic capabilities relevant to the COBRE Projects. This Core will support the young investigators by providing advanced analytical technologies and expertise in the metabolic requirements for cancer development and behavior.

项目概要 “代谢组学”主要包括代谢分析，这是识别和量化代谢物的过程。来自不同生物样品的许多不同生化类别的大量代谢物，包括然而，要了解疾病的代谢基础，例如细胞、培养基、组织和生物体液。与癌症一样，必须阐明生化机制，在这种情况下，有必要引入适当的方法虽然这大大增加了要定量的分析物的数量，但它也使得能够检索更多生物学相关信息。稳定同位素解析代谢组学 (SIRM)。方法是代谢核心的独特专长，由拥有 40 多年综合专业知识的 PI 领导 SIRM 的目标是为代谢组的广泛覆盖提供服务。为了实现这一目标，我们利用了同位素异构体和同位素异构体分析以及高通量蛋白质分析。四大分析平台，即（1）高分辨率核磁共振（NMR）、（2）超高分辨率傅立叶变换质谱 (UHR-FTMS)，带或不带色谱，(3) 超痕量通过电感耦合等离子体质谱仪 (ICPMS) 进行元素分析，以及 (4) 反相蛋白阵列 (RPPA)。代谢核心人员将就分析方法和基于 SIRM 的实验设计提供建议开发其他技术来解决识别和定量以及生物方面的具体问题与 COBRE 项目相关的解释，因为实验设计和样品制备是不可或缺的。对于数据采集和解释，代谢核心提供这些和信息学方面的专业知识这些目标将根据以下具体目标来实现。目标 1. 提供广泛的 NMR、MS 和 RPPA 技术的使用和专业知识具体目标 2. 为研究人员提供实验设计、样品处理和支持。研究人员进行数据缩减、代谢途径和生化机制分析。 3. 开发和实施新方法来增强与COBRE相关的代谢能力该核心项目将通过提供先进的分析技术和技术来支持年轻的研究人员。癌症发展和行为的代谢要求方面的专业知识。