INTERCELLULAR COMMUNICATION IN THE LENS

晶状体中的细胞间通讯

• 批准号: 2164569
• 负责人: Lisa Ebihara
• 金额: $ 17.2万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2164569
• 关键词: Xenopus; Xenopus oocyte; animal genetic material tag; cell cell interaction; chimeric proteins; divalent cations; electrophysiology; fluorescence microscopy; gap junctions; gene deletion mutation; gene expression; hydrogen; laboratory rat; lens; membrane channels; northern blottings; protein sequence; protein structure function; transfection; voltage /patch clamp

The long term objectives of the research proposal are to obtain detailed information about the functional properties of 3 lens fiber gap junctional proteins and the relationship between connexin structure and function. Detailed knowledge about these processes is important if we are to understand how the functional properties of different connexins contribute to organ homeostasis and how cataracts arise. There are 5 specific aims: 1. To characterize further the functional properties of 3 lens gap junctional proteins expressed in Xenopus oocyte pairs. 2. To characterize the behavior of single, nonjunctional cx46-induced channels in solitary oocytes. 3. To determine the role of the carboxyl terminal domain of cx46 and cx50 in channel function. 4. To determine the role of other protein domains in channel gating and permeation. 5. To characterize the functional properties of rat cx46 and rat cx50 in connexin-transfected mammalian cell lines using the dual patch clamp technique.

研究建议的长期目标是获得详细的目标 有关3个镜头纤维间隙连接的功能特性的信息 蛋白质与连接蛋白结构与功能之间的关系。 如果我们要 了解不同连接素的功能特性如何贡献 器官稳态以及白内障的产生。 有5个具体目标： 1。要进一步表征3个镜头间隙的功能特性 以爪蟾卵母细胞对表达的连接蛋白。 2。表征单个非功率CX46诱导的行为 孤立卵母细胞中的通道。 3。确定CX46和CX50的羧基终端结构域的作用 在通道功能中。 4。确定其他蛋白质结构域在通道门控和 渗透。 5。表征大鼠CX46和大鼠CX50的功能特性 使用双斑块夹连接连接的哺乳动物细胞系 技术。