Core B: Shared Resource Core For Characterizing Antibody Responses To SARS-CoV-2 And Other Pathogenic Human Coronaviruses

核心 B：用于表征对 SARS-CoV-2 和其他致病性人类冠状病毒的抗体反应的共享资源核心

• 批准号: 10222242
• 负责人: Aravinda M. DeSilva
• 金额: $ 52.88万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10222242
• 关键词: 2019-nCoV; Affinity; Antibodies; Antibody Response; Antigen Receptors; Antigens; B-Lymphocytes; Biological Assay; Containment; Coronavirus; Coronavirus spike protein; Detection; Enzyme-Linked Immunosorbent Assay; Exposure to; Fc Receptor; Goals; Gold; Health care facility; Immune; Immune Sera; Immunoglobulin Genes; Individual; Infection; Label; Length; Mammalian Cell; Measures; Methods; Middle East Respiratory Syndrome; N-terminal; North Carolina; Nucleocapsid; Paralysed; Pathogenicity; Pattern; Production; Property; Proteins; Recombinants; Research; Research Project Grants; Resource Sharing; SARS coronavirus; Science; Serologic tests; Serological; Serum; Severe Acute Respiratory Syndrome; Site; Sorting - Cell Movement; Specificity; Structure; System; Testing; Vaccines; Viral; Viral Pathogenesis; Virus; base; human coronavirus; milligram; pandemic disease; receptor binding

Abstract High-quality recombinant antigens and reliable and accurate SARS-CoV-2 serological assays are urgently needed for surveillance and for defining the immune mechanisms of viral pathogenesis, viral clearance, and protection against reinfection. Core B of the UNC SARS-CoV-2 Serological Sciences Center of Excellence will apply state-of-the-art strategies to establish methods for the production of milligram quantities of homogeneous recombinant spike protein CoV antigens with versatile tags and site-specific labels and use these antigens to develop sensitive and reliable singleplex and multiplex Ab detection assays. Core B will interact and support all the projects driven by the center and collaborators' by providing recombinant antigens and serological assays to characterize the serum antibody response, elucidate Fc effector functional profiles, and isolate and sequence antigen-specific immunoglobulin gene repertoire.

抽象的 迫切需要高质量的重组抗原和可靠、准确的 SARS-CoV-2 血清学检测 监测和确定病毒发病机制、病毒清除和免疫机制所需的 防止再次感染。北卡罗来纳大学 SARS-CoV-2 血清学科学卓越中心的核心 B 将 应用最先进的策略来建立毫克量均质产品的生产方法 具有多功能标签和位点特异性标签的重组刺突蛋白 CoV 抗原，并使用这些抗原 开发灵敏可靠的单重和多重抗体检测方法。核心B将交互并支持所有 该中心和合作者通过提供重组抗原和血清学检测来推动项目 表征血清抗体反应，阐明 Fc 效应子功能谱，并进行分离和测序 抗原特异性免疫球蛋白基因库。