HORMONAL REGULATION OF NUTRIENT TRANSPORT IN FISH INTESTINE

鱼肠中营养物质运输的激素调节

• 批准号: 3734333
• 负责人: GREGORY A. AHEARN
• 金额: --
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3734333
• 关键词: alternatives to animals in research; carbohydrate transport; dietary aminoacid; dietary carbohydrates; fish; gastrointestinal nutrient absorption; hormone regulation /control mechanism; insulin; radionuclides; short chain fatty acid; testosterone; tissue /cell culture

Over the past 5 years the laboratory of the applicant has been studying the effects of hormones on gastrointestinal absorption of dietary amino acids and sugars using epithelial cells of the teleost intestine. A variety of experimental approaches to this problem have been used during this period including electrophysiology, cell suspensions, purified vesicle techniques, and cell culture. All these studies have provided some insight into how a specific vertebrate hormone enhances the transintestinal absorption of a specific nutrient. To date, however, a complete picture of this mechanism is not available for any solute or any hormone in the lower vertebrates. The proposed investigation will use the combination of membrane vesicle techniques and epithelial cell culture procedures to ascertain how at least two hormones, insulin and alpha-methyltestosterone, influence the transintestinal absorption of a sugar, an amino acid, and a short chain fatty acid. To accomplish these goals, purified brush border membrane vesicles will be prepared according to standard methods we have employed for the last 10 years. These vesicles will be used to measure the properties of solute transport across this membrane in response to hormone stimulation. In addition, confluent epithelial monolayers grown in cell culture will be used to expose the intestinal cells to the respective hormones for predetermined times before assessing the possible effects of this exposure to transcellular movements of each organic solute. These studies taken together should allow us to determine the specific cellular and membrane effects of each hormone on the absorption of the selected dietary constituents.

在过去的5年中，申请人的实验室一直在研究 激素对饮食氨基胃肠道吸收的影响 使用硬骨肠上皮细胞的酸和糖。 一个 在此期间使用了多种实验方法 此期间包括电生理学，细胞悬浮液，纯化 囊泡技术和细胞培养。 所有这些研究提供了 对特定脊椎动物激素如何增强的一些洞察力 特定营养素的透脑吸收。 但是，迄今为止， 该机制的完整图片不适合任何溶质或任何溶质 脊椎动物中的激素。 拟议的调查将使用 膜囊泡技术和上皮细胞的组合 培养程序，以确定至少两种激素，胰岛素和 α-甲基睾丸激素，影响A的透视吸收 糖，氨基酸和短链脂肪酸。 完成这些 目标，纯化的​​刷子边框膜囊泡将根据 在过去10年中，我们采用的标准方法。 这些 囊泡将用于测量溶质传输的特性 响应激素刺激的整个膜。 此外， 在细胞培养中生长的汇合上皮单层将用于 将肠细胞暴露于相应的激素中以进行预先确定 在评估这种暴露的可能影响之前 每个有机溶质的跨细胞运动。 这些研究进行了 一起应该让我们确定特定的细胞和膜 每种激素对选定饮食的吸收的影响 成分。