60 HZ EMF EFFECTS ON TRANSCRIPTION IN YEAST

60 HZ EMF 对酵母转录的影响

• 批准号: 2156376
• 负责人: DAVID BINNINGER
• 金额: $ 13.44万
• 依托单位: FLORIDA ATLANTIC UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-28 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2156376
• 关键词: RNase protection assay; Saccharomyces cerevisiae; electromagnetic radiation; gene induction /repression; genetic transcription; messenger RNA; nucleic acid probes; nucleotide metabolism; radiation genetics; stress proteins; tissue /cell culture

potential health hazards associated with exposure to extremely low epidemiological studies that suggest a link between exposure to 60 Hz certain types of human cancers. However, a scientific answer to this question is not yet available because the molecular mechanisms that mediate cellular interactions with EMF are just beginning to be elucidated. The proposed experiments utilize Saccharomyces cerevisiae (yeast) as an eukaryotic model system because it provides elegant genetic and molecular cloning approaches that are not available for other types of cells. Specifically, they are interested in the molecular basis whereby 60 Hz EMF affect the expression of specific genes, presumably at the level of transcription. This biological phenomenon was first reported by Weisbrot et al. Toward further understanding of the molecular processes that mediate these effects on gene expression, they have three specific aims: 1) To rigorously replicate the published experiments cited above. These investigators reported that expression of two genes the SSA1 heat shock gene and URA3, a gene involved in nucleotide metabolism - is induced within 15-20 minutes on exposure of yeast to 60 Hz EMF. Then, within less than 60 minutes, transcript levels for both genes return to control levels; 2) To extend the investigation of these published experiments by examining how the molecular response is affected by the introduction of additional exposure parameters. For example, they will modify the published protocol by removing the mu-metal shielding without affecting the incubation conditions of the cells. This will allow them to evaluate directly whether exposure to any weak stray AC fields and the earth's magnetic fields alters the biological response observed when the cells were maintained within the Mu-metal chamber; and 3) To determine whether any of EMF-exposure parameters that influence transcriptional activity during short term EMF exposure also mediate this bioeffect during the longer exposure periods used in their yeast EMF-exposure protocol. Insight into the mechanism of action in yeast will shed light on the molecular basis of the responses that have been observed in more complex eukaryotic organisms. Thus, these studies will help fill the gap in their knowledge to evaluate more critically whether exposure to 60 Hz EMF present a health hazard to humans.

与暴露于极低浓度的环境有关的潜在健康危害 流行病学研究表明暴露于 60 Hz 频率之间存在联系 某些类型的人类癌症。 然而，这个问题有一个科学的答案 问题尚不明确，因为分子机制 介导细胞与 EMF 的相互作用才刚刚开始 阐明了。 拟议的实验利用酿酒酵母（酵母）作为 真核模型系统，因为它提供了优雅的遗传和分子 克隆方法不适用于其他类型的细胞。 具体来说，他们对 60 Hz 的分子基础感兴趣 EMF 影响特定基因的表达，大概在以下水平： 转录。 这种生物现象首先由 Weisbrot 报道 等人。 进一步了解介导的分子过程 这些对基因表达的影响，他们有三个具体目标：1） 严格复制上述已发表的实验。这些 研究人员报告说，SSA1 热休克两个基因的表达 基因和 URA3（一种参与核苷酸代谢的基因）被诱导 将酵母暴露于 60 Hz EMF 后 15-20 分钟内。 然后，在 不到 60 分钟，两个基因的转录水平就恢复到控制范围 级别； 2）扩大对这些已发表实验的研究 通过检查分子反应如何受到引入的影响 额外的曝光参数。 例如，他们将修改 通过移除高导磁合金屏蔽而不影响发布的协议 细胞的孵育条件。 这将使他们能够 直接评估是否暴露于任何微弱的杂散交流场以及 地球磁场改变了观察到的生物反应 细胞维持在高导磁合金室内； 3) 确定 是否有任何 EMF 暴露参数影响转录 短期 EMF 暴露期间的活动也会介导这种生物效应 在酵母 EMF 暴露中使用的较长暴露时间 协议。 深入了解酵母的作用机制将有助于揭示 在更复杂的情况下观察到的反应的分子基础 真核生物。 因此，这些研究将有助于填补这一空白 他们的知识可以更批判性地评估是否暴露于 60 Hz EMF 对人类健康构成危害。