Acquisition of a Beckman-Coulter Optima Analytical Ultracentrifuge

购买 Beckman-Coulter Optima 分析超速离心机

基本信息

批准号：
10176675
负责人：
KUSHOL GUPTA
金额：
$ 47.1万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-05 至 2022-04-04
项目状态：
已结题

项目摘要

Acquisition of a Beckman-Coulter Optima Analytical Ultracentrifuge Project Summary/Abstract: This proposal details a funding request for a Beckman-Coulter Optima analytical ultracentrifuge with multi- wavelength UV-Visible absorption and Rayleigh interference detection optics, with necessary accessories. The instrument will be installed in the Johnson Research Foundation Structural Biology and Biophysics Core (JRFSBBC) Facility that is overseen by the Department of Biochemistry and Biophysics at the Perelman School of Medicine of the University of Pennsylvania. The instrument will support NIH-funded research by investigators at Penn and other research institutions in the Greater Philadelphia region, collaborative research with NIH and NSF-funded researchers from across the US where similar technology and expertise is not available, and the training of graduate students and postdoctoral trainees. This new instrument will replace a ~25-year-old Beckman XL-A analytical ultracentrifuge (AUC) that currently serves the core. The new instrument represents an important advance in analytical ultracentrifugation and enables the application of arising methods that leverage multiwavelength data collection; this is currently not possible with the older instrument. These technical advances include improved wavelength reproducibility, vastly improved signal-to- noise, and faster scanning rates, which together will provide superior precision in the study of the thermodynamics of macromolecular associations and shape in solution. The research projects that will be advanced are broad in scope and all high-impact, including HIV drug discovery, cancer, antibiotic resistance, integral membrane protein drug targets, neurodegenerative disorders, and chromatin structure. The technology sought is relatively new, with few such instruments available in the academic setting nationwide (since early 2017) and is expected to impact a large group of intra- and extramural users. The instrument will be managed by the very experienced team at the JRFSBBC, which was established in 2016, within an already large and robust research environment.

购买 Beckman-Coulter Optima 分析超速离心机项目摘要/摘要：该提案详细介绍了贝克曼库尔特 Optima 分析超速离心机的资金申请，该离心机具有多个波长紫外-可见光吸收和瑞利干涉检测光学器件，以及必要的配件。该仪器将安装在约翰逊研究基金会结构生物学和生物物理学核心 (JRFSBBC) 由佩雷尔曼生物化学和生物物理学系监督的设施宾夕法尼亚大学医学院。该仪器将支持 NIH 资助的研究宾夕法尼亚大学和大费城地区其他研究机构的研究人员进行合作研究与美国国立卫生研究院 (NIH) 和国家科学基金会 (NSF) 资助的来自美国各地的研究人员合作，而这些地区没有类似的技术和专业知识可用，以及研究生和博士后培训生的培训。这种新仪器将取代目前为核心提供约 25 年历史的 Beckman XL-A 分析超速离心机 (AUC)。新的该仪器代表了分析超速离心的重要进步，并且能够应用利用多波长数据收集的新兴方法；这对于较旧的目前是不可能的乐器。这些技术进步包括改进的波长再现性、大大改进的信号到噪声和更快的扫描速率，这些共同将为研究提供卓越的精度溶液中大分子缔合和形状的热力学。将进行的研究项目先进的范围广泛且影响深远，包括艾滋病毒药物发现、癌症、抗生素耐药性、整合膜蛋白药物靶点、神经退行性疾病和染色质结构。这所寻求的技术相对较新，全国学术环境中很少有此类仪器（自 2017 年初以来）预计将影响一大批校内和校外用户。该仪器将由 JRFSBBC 经验丰富的团队管理，该团队成立于 2016 年，庞大而强大的研究环境。