Glacios 200 kV cryogenic transmission electron microscope (cryo-TEM)

Glacios 200 kV 低温透射电子显微镜 (cryo-TEM)

• 批准号: 10176876
• 负责人: THOMAS B THOMPSON
• 金额: $ 200万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176876
• 关键词: Address; Architecture; Area; Biological; Biology; Cardiovascular system; Collaborations; Communicable Diseases; Complex; Cryoelectron Microscopy; Crystallization; Electron Microscope; Electron Microscopy; Environment; Funding; Institution; Investments; Kentucky; Learning; Life; Malignant Neoplasms; Medical center; Microscope; Modernization; Molecular; Negative Staining; Neurosciences; Ohio; Pediatric Hospitals; Play; Postdoctoral Fellow; Protein Analysis; Research; Research Personnel; Resolution; Sampling; Structural Biologist; Structure; Techniques; Technology; Training; United States National Institutes of Health; Universities; X-Ray Crystallography; cryogenics; detector; graduate student; insight; instrument; instrumentation; macromolecule; programs; structural biology; transmission process

PROJECT SUMMARY Structural biology has played a key role in defining the molecular architecture of life, leading to fundamental insights into the function and biology of macromolecules. Within our Ohio Valley consortium, consisting of the University of Cincinnati, Cincinnati's Children's Hospital Medical Center, Miami University, and University of Kentucky, all have well-established programs in structural biology, with a major focus on X-ray crystallography. This has provided an outstanding training environment for graduate students and postdoctoral fellows to learn modern structural biology approaches to address fundamental questions around biomedically important macromolecules. However, in recent years, cryogenic electron microscopy (cryo-EM) has emerged as an indispensable technique for structural analysis of proteins and molecular complexes due to the fact that it can produce atom-resolution structures without drawbacks inherent to crystallization. Thus, our consortium structural biologists have been actively working to incorporate this technology into their research programs, primarily through collaboration and attempts to access instruments at other institutions. As a result of these efforts, we now have a critical mass of investigators that strongly justifies bringing this technology to our region. The University of Cincinnati has committed to laying the groundwork for this initiative by purchasing a Talos L120C instrument for negative stain analysis and a Vitrobot to prepare grids with a vitrified sample. These investments will allow users the ability to ascertain the quality of biological samples for further cryo-EM analysis. To take us to the next level, we are requesting NIH funding for a Glacios 200 kV microscope with Falcon 4 detector and autoloader, which will be housed in a newly developing cryo-EM facility at the University of Cincinnati. The Glacios will allow us to further optimize samples for structural analysis in house, or provide the justification for use of advanced cryo-EM instrumentation available at other institutions or NIH consortia for higher resolution studies. Collectively, the acquisition of the Glacios will directly benefit over 30 NIH funded investigators working in the focus areas of Cardiovascular, Cancer, Infectious Disease, and Neuroscience.

项目概要 结构生物学在定义生命的分子结构方面发挥了关键作用，导致了基本的 对大分子的功能和生物学的见解。在我们的俄亥俄谷财团内，包括 辛辛那提大学、辛辛那提儿童医院医学中心、迈阿密大学和辛辛那提大学 肯塔基州都拥有完善的结构生物学项目，主要侧重于 X 射线晶体学。 这为研究生和博士后学习提供了良好的培养环境 现代结构生物学方法解决生物医学重要的基本问题 大分子。然而，近年来，低温电子显微镜（cryo-EM）已成为一种 蛋白质和分子复合物结构分析不可或缺的技术，因为它可以 产生原子分辨率结构，没有结晶固有的缺点。因此，我们的财团 结构生物学家一直在积极努力将这项技术纳入他们的研究计划， 主要通过合作和尝试获取其他机构的工具。由于这些 经过努力，我们现在拥有足够数量的研究人员，有力地证明了将这项技术引入我们地区的合理性。 辛辛那提大学致力于通过购买 Talos 为这一举措奠定基础 用于负染色分析的 L120C 仪器和用于制备带有玻璃化样品的网格的 Vitrobot。这些 投资将使用户能够确定生物样品的质量以用于进一步的冷冻电镜 分析。为了让我们更上一层楼，我们请求 NIH 资助 Glacios 200 kV 显微镜， Falcon 4 探测器和自动装载机将安装在大学新开发的冷冻电镜设施中 辛辛那提。 Glacios 将使我们能够进一步优化内部结构分析的样品，或提供 使用其他机构或 NIH 联盟提供的先进冷冻电镜仪器的理由 更高分辨率的研究。总的来说，收购 Glacios 将直接惠及超过 30 家 NIH 资助的机构 研究人员在心血管、癌症、传染病和神经科学等重点领域工作。