Transcriptomics and Repertoire Profiling

转录组学和谱库分析

基本信息

批准号：
10176370
负责人：
Peter Alan Sims
金额：
$ 48.18万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-06-25 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10176370
关键词：
ATAC-seq Academic Medical Centers Antibodies Antibody Repertoire Architecture B-Lymphocyte Subsets B-Lymphocytes Bar Codes Biological Assay Cells ChIP-seq Chromatin Chromium Cloning Collaborations Complementary DNA Core Facility DNA Sequencing Facility DNA sequencing Data Data Analyses Epigenetic Process Equipment Expression Profiling Flow Cytometry Flow Cytometry Shared Resource Gene Rearrangement Generations Genes Genetic Transcription Genomics Goals Human Immune Immunology Letters Libraries Liquid substance Logistics Lymphocyte Maintenance Measures Messenger RNA Methods Microfluidics Molecular Molecular Analysis Pennsylvania Performance Population Procedures Protocols documentation Research Personnel Resources Reverse Transcription Robot Running Sampling Services Sorting - Cell Movement System T-Cell Receptor T-Lymphocyte T-cell receptor repertoire Technology Testing Tissues Universities Validation base epigenomics experience innovation instrument instrumentation new technology next generation sequencing programs single cell analysis single-cell RNA sequencing technology development transcriptome transcriptome sequencing transcriptomics

项目摘要

CORE C: PROJECT SUMMARY The main goal of this core is to establish and provide robust experimental procedures for profiling the transcriptomes and immune repertoires of tissue-resident lymphocytes. We will employ innovative methods for whole transcriptome and immune repertoire profiling in bulk populations, epigenomics, and single cell analysis. For low-input, population-level RNA-Seq we will use our recently established protocol for high-sensitivity mRNA capture and library construction. We will also provide bulk ATAC-Seq for measuring open chromatin. For single- cell RNA-Seq, we will deploy our microfluidic system for large-scale single cell capture and cDNA barcoding as well as a highly efficient plate-based method for whole transcriptome amplification. Finally, for dissecting the clonal architecture of tissue-resident T and B cells, we will provide cutting-edge methods for T cell receptor and antibody repertoire profiling.

核心 C：项目摘要该核心的主要目标是建立并提供强大的实验程序来分析组织驻留淋巴细胞的转录组和免疫库。我们将采用创新方法大量群体的全转录组和免疫组库分析、表观基因组学和单细胞分析。对于低输入、群体水平的 RNA 测序，我们将使用我们最近建立的高灵敏度 mRNA 协议捕获和库构建。我们还将提供批量 ATAC-Seq 用于测量开放染色质。对于单细胞 RNA-Seq，我们将部署我们的微流体系统进行大规模单细胞捕获和 cDNA 条形码以及一种高效的基于平板的全转录组扩增方法。最后，为了剖析组织驻留 T 细胞和 B 细胞的克隆结构，我们将为 T 细胞受体和抗体库分析。