REGULATION OF PROLACTIN STORAGE

催乳素储存的调节

• 批准号: 2146053
• 负责人: PRISCILLA S DANNIES
• 金额: $ 12.14万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-15 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2146053
• 关键词: chimeric proteins; fusion gene; genetic manipulation; glycosylation; granule; human genetic material tag; posttranslational modifications; prolactin; protein sequence; protein structure function; secretory protein; somatotropin

DESCRIPTION (Adapted from the applicant's abstract): The mechanisms by which proteins are packaged into secretory granules are not understood. Two models that have been proposed are l) self-association or aggregation and 2) recognition of a sorting sequence. These models have not been distinguished experimentally. In GH cells transfected with DNA sequences for human growth hormone, estradiol increases storage of rat prolactin but does not affect storage of human growth hormone. In GH cells transfected with DNA sequences for human prolactin, estradiol does not induce the storage of either rat or human prolactin, although both are still synthesized and secreted. These three molecules are structurally similar. This project will substitute portions of these hormones into each other to determine which amino acids are necessary to allow regulation of storage, and test specific models for packing prolactin into secretory granules.

描述（改编自申请人的摘要）：机制 哪些蛋白质被包装成分泌颗粒尚不清楚。 已提出的两种模型是 l) 自关联或 聚合和2）排序序列的识别。这些型号有 没有通过实验来区分。在转染 DNA 的 GH 细胞中 人类生长激素、雌二醇的序列增加了大鼠的储存 催乳素但不影响人类生长激素的储存。在GH 转染人催乳素、雌二醇 DNA 序列的细胞 不诱导大鼠或人催乳素的储存，尽管两者 仍然合成和分泌。这三个分子是 结构相似。该项目将替代其中的部分内容 激素相互结合以确定哪些氨基酸是必需的 允许储存调节，并测试特定型号的包装 催乳素变成分泌颗粒。