Bruker timsTOF pro LC-MS system

布鲁克 timsTOF pro LC-MS 系统

• 批准号: 10175940
• 负责人: Oliver Fiehn
• 金额: $ 59.99万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-15 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175940
• 关键词: Biogenic Amines; Chemicals; Collaborations; Communities; Dietary Intervention; Environmental Health; Funding; Infrastructure; Isomerism; Lipids; Methods; Minor; Organelles; Performance; Research; Resolution; Rett Syndrome; Structure; System; Technical Expertise; Work; base; gut microbiome; gut-brain axis; improved; instrument; instrumentation; ion mobility; lipidomics; mass spectrometer; metabolomics; oxidized lipid; polyphenol; screening; time use; tumor metabolism; urinary

Project Summary_Abstract We are applying for funds for a Bruker timsTOF Pro trapped ion mobility mass spectrometer LC- MS system. This LC-ion mobility MS systems offers superior resolution to alternative systems providing much deeper metabolomics, lipidomics and exposome screening capabilities than classic LC-accurate mass MS/MS instruments. With the enhanced performance of the requested Bruker timsTOF Pro LC-MS system, structurally similar lipids and other compounds can be accurately detected and identified. Such system is urgently needed to fill gaping unmet needs by the biomedical community here at UC Davis. We have identified 8 major users for this instrument for 80% of time usage and suggest a 20% time usage by the UC Davis metabolomics core to work on recharge-based projects for minor users. By providing our infrastructure and technical expertise on LC-MS metabolomics, we project a diverse and long-term collaborations with our users. The common thread across all projects is that chemical complexities are too large to be resolved ‘in depths’ by current LC-accurate mass MS/MS alone. Users need to get to the next stage to solve their research problems adequately. Projects that need for ion mobility are such as: environmental health (Young: deeper MS/MS coverage for non-targeted screening, van Winkle: higher resolution for oxidized lipids), cancer metabolism (Carraway: improved sensitivity and resolution for BMP and PG lipidomics in organelles, Chen: improved resolution of oxysterol isomers), diurnal cycles (Chiu: resolving isobaric and isomeric triacylglycerides), nutritional interventions (Stanhope: resolving urinary polyphenols), methods in compound identification (Fiehn: improving confidence in structure annotations by CCS values) to gut microbiome changes (LaSalle: interaction of gut-brain axis in Rett syndrome, resolution of biogenic amines in fecal matter). All these projects have in common that they have hit roadblocks in our ongoing collaborations with respect to capabilities of our current instrumentation at the WCMC that lack the added power of ion mobility separation despite our exquisite capabilities in accurate mass analyses.

项目概要_摘要 我们正在申请资金购买布鲁克 timsTOF Pro 离子淌度质谱仪 LC- 该 LC 离子淌度 MS 系统提供优于替代系统的分辨率。 提供比其他更深入的代谢组学、脂质组学和暴露组筛选能力 经典的 LC 精确质量 MS/MS 仪器具有所需的增强性能。 Bruker timsTOF Pro LC-MS 系统，结构相似的脂质和其他化合物可 迫切需要这样的系统来填补未满足的需求。 加州大学戴维斯分校的生物医学界我们已经确定了该仪器的 8 个主要用户。 80% 的时间使用，并建议 UC Davis 代谢组学核心使用 20% 的时间 致力于针对未成年人用户的充值项目。 通过提供我们在 LC-MS 代谢组学方面的基础设施和技术专业知识，我们计划 与我们的用户进行多样化和长期的合作是所有项目的共同点。 化学复杂性太大，无法通过当前的 LC 精确质量数“深入”解析 用户需要进入下一阶段才能充分解决他们的研究问题。 需要离子淌度的项目如：环境健康（Young：更深层次的 MS/MS 非靶向筛查的覆盖范围、van Winkle：更高分辨率的氧化脂质）、癌症 代谢（Carraway：提高了 BMP 和 PG 脂质组学的灵敏度和分辨率 细胞器，Chen：提高了氧甾醇异构体的分辨率），昼夜循环（Chiu：分辨率 同量和异构三酰甘油酯）、营养干预（Stanhope：解决尿问题） 多酚），化合物鉴定方法（Fiehn：提高结构的可信度 CCS 值的注释）对肠道微生物组变化的影响（LaSalle：肠道-大脑轴的相互作用 雷特综合征，粪便中生物胺的解决）所有这些项目都有共同点。 他们在我们持续的合作中遇到了障碍 WCMC 目前的仪器缺乏离子淌度分离的附加功能，尽管 我们在精确质量分析方面的精湛能力。