UROLITHIASIS--ROLE OF NEPHRON DYSFUNCTION/INJURY

尿石症——肾单位功能障碍/损伤的作用

• 批准号: 2142832
• 负责人: MANI MENON
• 金额: $ 24.43万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2142832
• 关键词: acid base balance; bicarbonates; biological signal transduction; calcium flux; cell membrane; chlorine; disease /disorder model; hypercalciuria; kidney cell; laboratory rat; nephrolithiasis; oxalates; renal tubule; tissue /cell culture; urinary calculi

Oxalate, an end product of metabolism, is a major component of 80% of kidney stones, and small changes in its urinary concentration critically influence calcium oxalate crystallization. The high frequency of a family history in "idiopathic" renal stone disease has suggested to some investigators that urolithiasis may be a metabolic disorder characterized by a defect in cellular oxalate transport. Inherited defects in erythrocyte calcium and oxalate transport have been recently identified in patients with kidney stones, strengthening the contention that abnormalities in cellular transport may play a primary role in "idiopathic" calcium stone formation. However, the cellular transport of oxalate has not been characterized in the kidney, particularly in papillary cells where calcium oxalate crystallization usually occurs. The aims of this proposal, thus are; 1. To characterize oxalate transport in renal cortical and papillary cells. 2. To assess alterations in oxalate handling in experimental models of nephrolithiasis that feature nephron dysfunction or injury. These studies will help elucidate the role of nephron dysfunction or injury in the pathogenesis of calcium oxalate nephrololithiasis and may give us insights on how to prevent this disease.

草酸盐是代谢的最终产物，是80％的主要成分 肾结石，其尿液浓度很小 影响草酸钙结晶。 家庭的高频 “特发性”肾结石疾病的历史已向某些人提出 调查人员认为尿石症可能是一种代谢性疾病 由于细胞草酸盐转运的缺陷。 继承的缺陷 最近在 肾结石的患者，加强了 细胞运输的异常可能在“特发性”中起主要作用 钙石的形成。 但是，草酸盐的细胞转运有 没有在肾脏中表征，特别是在乳头细胞中 草酸钙结晶通常发生。 因此，该提议的目的是； 1。表征在肾皮质和乳头状中的草酸盐转运 细胞。 2。评估在实验模型中草酸盐处理的改变 具有肾单位功能障碍或损伤的肾结石病。 这些研究将有助于阐明肾单位功能障碍或损伤的作用 在草酸钙肾石岩症的发病机理中，可能会给我们 关于如何预防这种疾病的见解。