Molecular anatomy resources for postembryonic zebrafish

胚胎后斑马鱼的分子解剖资源

• 批准号: 10170587
• 负责人: DAVID M PARICHY
• 金额: $ 8.08万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-10 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10170587
• 关键词: Adult; Anatomy; Animal Model; Atlases; Biomedical Research; Cells; Communities; Congenital Abnormality; Data; Databases; Development; Disease; Embryo; Event; Fishes; Health; Human; In Situ Hybridization; Investigation; Laboratories; Larva; Location; Messenger RNA; Molecular; Morphogenesis; Natural regeneration; Organ; Organogenesis; Pattern; Phenotype; Process; Research; Research Personnel; Resources; Spatial Distribution; System; Tissues; Work; Zebrafish; candidate marker; cell type; diagnostic biomarker; histological image; human disease; imaging approach; molecular marker; mutant; regeneration potential; single-cell RNA sequencing; transcriptomics; zebrafish development

PROJECT SUMMARY The zebrafish is a widely used biomedical model organism and has been studied extensively at early stages. Current research efforts focus increasingly on later stages that are relevant to understanding fundamental as- pects of vertebrate development as well as human disease, birth defects and regenerative potential. This project will provide a resource to facilitate such studies by identifying molecular markers for specific cell types present at late stages, and mapping these markers and the cells they represent within the developing anatomy of the larval fish. Candidate markers and cell types will be identified using unbiased single cell transcriptomic approaches, and anatomical mapping will be accomplished using histological and imaging approaches, all of which are routine for this laboratory. These resources will enable efforts by the community of investigators us- ing zebrafish for biomedical research, and will provide opportunities to further exploit this system for under- standing and promoting human health.

项目概要 斑马鱼是一种广泛使用的生物医学模型生物，并在早期阶段得到了广泛的研究。 当前的研究工作越来越关注与理解基本原理相关的后期阶段： 脊椎动物发育以及人类疾病、出生缺陷和再生潜力的影响。 该项目将通过识别特定细胞类型的分子标记来提供资源来促进此类研究 存在于晚期阶段，并绘制这些标记及其在发育中的解剖结构中代表的细胞 幼鱼的候选标记和细胞类型将使用无偏单细胞转录组学进行鉴定。 方法和解剖图绘制将使用组织学和成像方法来完成，所有 这些资源是该实验室的常规资源，将有助于研究人员的努力。 荷兰国际集团将斑马鱼用于生物医学研究，并将为进一步利用该系统提供机会 站立并促进人类健康。